| Summary: | 博士 === 國立陽明大學 === 醫學工程研究所 === 87 === The aim of this study is to prepare a collagen-based microcapsule for cell culture using alginate as an encapsulation medium. Furthermore, theoretical modeling has been conduct to simulate the concentration distribution of growth factor within the cell culture matrix. Collagen is the major structural protein in the extracellular matrices of connective tissues. With proper treatment, its immune response can be eliminated and provide a better biocompatibility. Purified collagen molecules can be reconstituted into a three-dimensional fibrous network at 37℃, pH = 7.4. Present commercialized products of collagen beads are obtained by quenched in liquid nitrogen and then crosslinked with glutaraldehyde. Such kind of gel beads can''t be applied as a biomaterial directly because of its poor biocompatibility. We introduce a method of preparing microcapsules containing collagen fibrous network. This method takes the advantage of the gelation properties of alginate and forms spherical gel beads by dropping a mixture of collagen and alginate into a solution of CaCl2 at 4℃. Collagen was then reconstitute within the microcapsules at 37℃ after alginate was liquefied with citrate. Both of the growth oh the GH3 rat pituitary tumor cell and the L929 fibroblastic-like cell were studied for the present microcapsules. The results indicate that both cells proliferate faster in the collagen-containing capsule than those of in the conventional collagen-free microcapsules. It is believed that by applying specific growth factor, the cell proliferation rate can be promoted. In this study, a mass transfer model is developed to simulate the concentration distribution of growth factor molecules. The established model can be applied in designing a cell culture material.
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