| Summary: | 碩士 === 中國醫藥學院 === 中國醫學研究所 === 88 === Studies were conducted to examine the dose effects of luteolin on N-acetylation of 2-aminofluorene (2-AF) from normal Spraque-Dawley rat’s tissure and human liver tumor cells（J-5）. For cytosol examination, the effects on the N-acetylation of 2-AF were determined by using acetyl Co-A recycling assay and high pressure liquid chromatography. For intact cells examination, the 2-AF were placed into the culture then to assay the amounts of acetylated 2-AF (N-acetyl-2-Aminofluorene: 2-AAF) and non-acetylated 2-AF. Cytosols or suspensions of rat’s tissues (blood and liver) and lymphocyte and human liver tumor cells with or without selected concentrations of luteolin co-treated showed different percentage of 2-AF and 2-AAF. The data indicates that there was a decreased N-acetylation of 2-AF associated with increased levels of luteolin in the cytolsols or suspension of examined tissues or cells.
Luteolin was also used to determined the cytotoxicity, cell morphology, apoptosis and cell cycle of human liver tumor cells（J-5）. The results show luteolin did induced cytotoxicity, the change of cell morphology, apoptosis and the change of cell cycle , especially decreased the number of S phase in J-5 cells. The cell cycle was based on the DNA content by using Flow cytometry analysis. This is the first finding to show that luteolin inhibit N-acetylation of 2-AF and affect cell growth from human liver tumor cells.
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