Mutation Analysis of the PPP2R1B Gene in Cervical Cancer

• Main Authors: Lian-Shung Yeh, 葉聯舜
• Other Authors: Fuu-Jen Tsai
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/80900487818619505452

碩士 === 中國醫藥學院 === 醫學研究所 === 88 === 英文摘要 Abstract The PPP2R1B gene encodes the b isoform of the A subunit of the serine/threonine protein phosphatase 2A (PP2A). PP2A holoenzyme plays a critical role in cell-cycle control and growth-factor signaling. The PPP2R1B gene is located on human chromosome 11q22-24 where loss of heterozygosity (LOH) was frequently observed in many carcinomas. LOH is a very important sign for analyzing the deletion of tumor suppressor genes, which lead to the development of a variety of human malignancies. Hence, PPP2R1B is a putative tumor suppressor gene and its alterations may be related to tumor development. Recently, the PPP2R1B gene somatic alterations were found in primary lung tumors, lung-derived cell line and primary colon tumors. In this study, we used nested RT-PCR and direct sequencing to analyzing 24 cases of cervical cancers and paired non-cancerous near-by tissues. The results showed that aberrant transcripts were found 5/24 cases of cancerous tissues, and 8/24 cases of non-cancerous tissues. We suggest that these aberrant transcripts play no role in the carcinogenesis of cervical cancer, and we also sequenced the whole cDNA of RT-PCR product of PPP2R1B, no point mutation or small-bases change in these 24 cervical cancers. We suggest that PPP2R1B may not play a role in the development of cervical cancer.