The effect catalpol on 2-aminofluore N-acetylation from human tumor cells in vitro and 2-aminofluore metabolites in Spregure-Dawley rats in vivo
碩士 === 中國醫藥學院 === 醫學研究所 === 88 === Catalpol, a natually occuring in plant Rehmannia glutionsa , has been demonstrated to pesses anti-cancer and anti-blood pressure function in human. There is no any available information to address the effect of catapol on cell cycle, DNA damage, and N-acetyltransf...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2000
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| Online Access: | http://ndltd.ncl.edu.tw/handle/33041522447835078552 |
| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 88 === Catalpol, a natually occuring in plant Rehmannia glutionsa , has been demonstrated to pesses anti-cancer and anti-blood pressure function in human. There is no any available information to address the effect of catapol on cell cycle, DNA damage, and N-acetyltransferance (NAT) activity in human liver tumor cell line. Therefore, the purpose of this study to be to determine whether Catalpol could affect human liver tumor cells.
Catapol was used to determine the inhibition of cell growth (J-5 cell) by trypin blue exclusion and revealed catalpol inhibite human liver tumor cell proliferation and chromose damage in this examined tumor cells. The DNA content (cell cycle) anyalsis in J-5 cells was determined by flow cytomertry. The result also show that the treatment of catalpol in cell culture results in the inhibtion of S phase in cell cycle of J-5 cells. The proportion cells in S phase progressive decreasing concentration with and increasing G phase cells. The degration of genomic DNA was also analyzed in reponse to catalpol treatment by size fraction in J-5 cells. This fingings is first demonstration to show that catapol decrease NAT activity and affect cell cycle of human liver tumor cells.
Dietary treatment of catalpol to the rat indicates that catalpol can decrease 2-aminofluore actylation and metabolism in vivo. The result also shows catalpol effect the distribution of 2-AF metabolites in rat different organs.
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