Investigation of human polyomavirus infection in individuals of various ages in TaiwanInvestigation of correlation between human polyomavirus and central nervous system tumors

• Main Authors: Hui-Sheng Lin, 林惠生
• Other Authors: Deching Chang
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/77173049741642641340

碩士 === 中山醫學院 === 生物化學研究所 === 88 === Abstract To investigate the prevalence of human polyomavirus in normal population with various ages in Taiwan, 874 urine samples were collected for analysis. Polymerase chain reaction (PCR) and DNA endonuclease digesting were performed to detect the viral DNA and identify genotype. The results showed that 1% (1/104) of age range 3-7, 3-2% (7/216) of age range 8-13, 6.5% (17/261)，13.3% (10/75) of age range 20-30, 30.0% (6/20) of age range 31-50, 41.7% (5/12) of age range 51-60, 45.1% (37/82) of age range 60-70 and 58.7% (61/104) of age greater than 70 were JCV positive. The results indicate that the incidence of JC viruria is increased with age, which may be correlated with immunity. In addition, CY (44.8%) and TW-1 (55.2%) are the predominant genotypes of JCV prevalent in Taiwan. Prevalence of human polyomavirus in Bunun aboriginal tribe was also investigated. Urine sample were collected for virus genotype analysis. Blood sample was used for anti-JC antibody determination by hemagglutination inhibition assay. The results showed that JC viruria were 50.8% (68/134), and anti-JC antibody positivity were 91.2% (124/136). The predominance of CY genotype [91.2% (62/68)] were more than TW-1 genotype [8.8% (6/68)] in Bunun tribe. Abstract Brain tumors represent near 2% of human neoplasm is preferentially affect children and older adults. The etiology of brain tumors in humans remains unknown. Human neurotropic JC virus is able to infect human brain glial cells. The causative agent of the fatal human demyelinating disease progressive multifocal leukoencephalopathy(PML), that cytolyses and destroys oligodendrocytes, the myelin-producing cells of the central nervous system. Also results from several experiments have indicated the JCV is able to induce neoplasms. Transgenic mice expressing the JCV early protein, T-antigen, develop neoplasms that exhibit a phenotype similar to that of human glial-derived tumors. Several case reports have detected JCV with human CNS tumors in patients with or without concomitant PML. In this study, we have examined the possible association of JCV with human brain tumor. We collected 47 specimens of brain tumor and 9 brain non-neoplasms specimens. By using pathological diagnosis in Hematoxylin-Eosion stain with auxiliary confirm immunohistochemistry stain of tissue section. Immunochemistry stain was used for viral early protein T-antigen. Positive samples included 11 of 29 glial-derived tumors, 3 of 11 metastatic tumors, and 3 of 9 non-neoplasms change. All of 7 non-glial-derived tumors showed negative results. These results provided evidence for a possible association of JCV T-antigen with glial-derived tumors and metastatic tumors. In addition, samples with non-neoplasm showed JCV LT positive in some astrocytes.