The investigation of the mechanism of decidualization in the ras

• Main Authors: Hui-Chen Liu, 劉惠珍
• Other Authors: Jer-Yuh Liu
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/20873636679272067209

碩士 === 中山醫學院 === 生物化學研究所 === 88 === we previous found that the PKC isoforms was involved in the development of the deciduomata in pseudopregnant rats .Therefore, in this study ,we determined the expression of protein kinase C ( PKC ) isoforms during pregnancy . The result showed that PKC α was down-modulation in the decidual cells but not in the myometrium . Down- modulation and were activation compatible with the increase in cell mitosis , which reached a maximum at 8-9 days. Contradictorily, PKC ζ was not down-modulated. It was increased both in the cytosolic and particulate fractions of the decidual cell , and paralleled with the frequency of decidual cells mitosis. The other PKC isoform of dulta was also increased , but it was associated with the cell regression . Immunohistochemistry assay confimed the activation of PKCalpha, with the perinuclear location but only on the extra circycle regien of the implantation site. Moreover, after the treated of diphenhydramine, it reduced the decidualization, and depleted the PKCα and PKCζ after 4 and 8 hour operation. But it was reversed by cotreated with PKC inhibitor, staurosporine and H7. Therefore, these findings suggested that the variablel expressions of PKC isoforms in decidualization tissue might be involved in the modulation of decidual cells growth, and the PKC isoforms were also associated with the inhibitory effect of diphenhydramine.