洛神花色素成分及其抗氧化活性之分析
碩士 === 中山醫學院 === 生物化學研究所 === 88 === Increasing evidence of free-radical generating agents and inflammatory process suggests accumulation of reactive oxygen species involving hepatotoxicity.In this study,we use tert-butylhydroperoxide(t-BHP) tto induce hepatotoxicity in vitro and in vivo to assess th...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2000
|
Online Access: | http://ndltd.ncl.edu.tw/handle/75120677157167488827 |
id |
ndltd-TW-088CSMC0107017 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-088CSMC01070172015-10-13T11:50:52Z http://ndltd.ncl.edu.tw/handle/75120677157167488827 洛神花色素成分及其抗氧化活性之分析 Jiunn-Min Wang 王俊民 碩士 中山醫學院 生物化學研究所 88 Increasing evidence of free-radical generating agents and inflammatory process suggests accumulation of reactive oxygen species involving hepatotoxicity.In this study,we use tert-butylhydroperoxide(t-BHP) tto induce hepatotoxicity in vitro and in vivo to assess the antioxidative bioactivity of color extracts of dried Hibiscus sabdariffa flowers(HS-colors).The investigations showed that pretreated-HS-colors(0.05-0.10mg/ml) significantly decreased the leakage of lactate dehydrogenase(LDH)''alanine aminotransaminase(ALT)''aspartatee aminotransferase(AST) and the formation of mmalondialdehyde(MDA) induced by 30 min treatment with t-BHP in primary cultured rat hepatocytes. In vivo study(rats), histopatthological evaluation of rat liver showed that groups treated with HS-colors at concentration of 50-200mg/kg for 5 day orally then with single dose of t-BHP(ip;0.2mmol/kg) reduced the incidence of liver lesion including inflammatory leukocytes infiltration and necrosis. Furthermore, groups pretreatted HS-colors at concentration of 50-200mg/kg for 5 day orally decerased the serum index(LDH''ALT''AST)of hepatic function and liver lipid peroxidation induced by t-BHP. In addition to scavenging free radical property,HS-colors might exert its protective effect through modulating intracellular detoxication enzyme such as catalase. Tsui-Hwa Tseng Chia-Yih Chu 曾翠華 朱嘉一 2000 學位論文 ; thesis 91 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 中山醫學院 === 生物化學研究所 === 88 === Increasing evidence of free-radical generating agents and inflammatory process suggests accumulation of reactive oxygen species involving hepatotoxicity.In this study,we use tert-butylhydroperoxide(t-BHP) tto induce hepatotoxicity in vitro and in vivo to assess the antioxidative bioactivity of color extracts of dried Hibiscus sabdariffa flowers(HS-colors).The investigations showed that pretreated-HS-colors(0.05-0.10mg/ml) significantly decreased the leakage of lactate dehydrogenase(LDH)''alanine aminotransaminase(ALT)''aspartatee aminotransferase(AST) and the formation of mmalondialdehyde(MDA) induced by 30 min treatment with t-BHP in primary cultured rat hepatocytes. In vivo study(rats), histopatthological evaluation of rat liver showed that groups treated with HS-colors at concentration of 50-200mg/kg for 5 day orally then with single dose of t-BHP(ip;0.2mmol/kg) reduced the incidence of liver lesion including inflammatory leukocytes infiltration and necrosis. Furthermore, groups pretreatted HS-colors at concentration of 50-200mg/kg for 5 day orally decerased the serum index(LDH''ALT''AST)of hepatic function and liver lipid peroxidation induced by t-BHP. In addition to scavenging free radical property,HS-colors might exert its protective effect through modulating intracellular detoxication enzyme such as catalase.
|
author2 |
Tsui-Hwa Tseng |
author_facet |
Tsui-Hwa Tseng Jiunn-Min Wang 王俊民 |
author |
Jiunn-Min Wang 王俊民 |
spellingShingle |
Jiunn-Min Wang 王俊民 洛神花色素成分及其抗氧化活性之分析 |
author_sort |
Jiunn-Min Wang |
title |
洛神花色素成分及其抗氧化活性之分析 |
title_short |
洛神花色素成分及其抗氧化活性之分析 |
title_full |
洛神花色素成分及其抗氧化活性之分析 |
title_fullStr |
洛神花色素成分及其抗氧化活性之分析 |
title_full_unstemmed |
洛神花色素成分及其抗氧化活性之分析 |
title_sort |
洛神花色素成分及其抗氧化活性之分析 |
publishDate |
2000 |
url |
http://ndltd.ncl.edu.tw/handle/75120677157167488827 |
work_keys_str_mv |
AT jiunnminwang luòshénhuāsèsùchéngfēnjíqíkàngyǎnghuàhuóxìngzhīfēnxī AT wángjùnmín luòshénhuāsèsùchéngfēnjíqíkàngyǎnghuàhuóxìngzhīfēnxī |
_version_ |
1716849421964017664 |