DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA
碩士 === 中山醫學院 === 醫學研究所 === 88 === ABSTRACT Little progress has been made in decreasing lung cancer mortality by applying conventional methods to early diagnosis and screening. Recent advances in molecular oncology, however, have provided tools which may be of use in this area....
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2000
|
Online Access: | http://ndltd.ncl.edu.tw/handle/86266829528726536384 |
id |
ndltd-TW-088CSMC0534004 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-088CSMC05340042015-10-13T11:50:52Z http://ndltd.ncl.edu.tw/handle/86266829528726536384 DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA 非小型細胞肺癌病人診斷前的痰抹片中偵測p53基因突變 Jung-Ta Chen 陳榮達 碩士 中山醫學院 醫學研究所 88 ABSTRACT Little progress has been made in decreasing lung cancer mortality by applying conventional methods to early diagnosis and screening. Recent advances in molecular oncology, however, have provided tools which may be of use in this area. p53 gene mutation is the most common gene alteration in the development of lung cancer. Conventional cytologic analysis of sputum is an insensitive test for the diagnosis of lung cancer. In this study, we attempted to establish a polymerase chain reaction (PCR)-based assay for assessing the possibility of early detection of p53 mutation in archival Papanicolaou-stained cytologic sputum smears. Ten sputum smear slides were collected prior to clinical diagnosis from 10 lung cancer patients who has been confirmed to have p53 mutations in surgically resected lung tumors. We successfully obtained sufficient amounts of RNA from each sputum smear specimen for amplification of PCR and direct sequencing. Only one patient was found to have p53 mutation at codon 286; the other nine patients had wild type p53 genes. This result supports the possibility that detection of p53 mutations in cytologic sputum smears is an available strategy for the early diagnosis of lung cancer. Huei Lee 李 輝 2000 學位論文 ; thesis 33 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 中山醫學院 === 醫學研究所 === 88 === ABSTRACT
Little progress has been made in decreasing lung cancer mortality by applying conventional methods to early diagnosis and screening. Recent advances in molecular oncology, however, have provided tools which may be of use in this area. p53 gene mutation is the most common gene alteration in the development of lung cancer. Conventional cytologic analysis of sputum is an insensitive test for the diagnosis of lung cancer. In this study, we attempted to establish a polymerase chain reaction (PCR)-based assay for assessing the possibility of early detection of p53 mutation in archival Papanicolaou-stained cytologic sputum smears. Ten sputum smear slides were collected prior to clinical diagnosis from 10 lung cancer patients who has been confirmed to have p53 mutations in surgically resected lung tumors. We successfully obtained sufficient amounts of RNA from each sputum smear specimen for amplification of PCR and direct sequencing. Only one patient was found to have p53 mutation at codon 286; the other nine patients had wild type p53 genes. This result supports the possibility that detection of p53 mutations in cytologic sputum smears is an available strategy for the early diagnosis of lung cancer.
|
author2 |
Huei Lee |
author_facet |
Huei Lee Jung-Ta Chen 陳榮達 |
author |
Jung-Ta Chen 陳榮達 |
spellingShingle |
Jung-Ta Chen 陳榮達 DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
author_sort |
Jung-Ta Chen |
title |
DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
title_short |
DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
title_full |
DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
title_fullStr |
DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
title_full_unstemmed |
DETECTION OF p53 MUTATIONS IN SPUTUM SMEARS PRECEDES DIAGNOSIS OF NON-SMALL CELL LUNG CARCINOMA |
title_sort |
detection of p53 mutations in sputum smears precedes diagnosis of non-small cell lung carcinoma |
publishDate |
2000 |
url |
http://ndltd.ncl.edu.tw/handle/86266829528726536384 |
work_keys_str_mv |
AT jungtachen detectionofp53mutationsinsputumsmearsprecedesdiagnosisofnonsmallcelllungcarcinoma AT chénróngdá detectionofp53mutationsinsputumsmearsprecedesdiagnosisofnonsmallcelllungcarcinoma AT jungtachen fēixiǎoxíngxìbāofèiáibìngrénzhěnduànqiándetánmǒpiànzhōngzhēncèp53jīyīntūbiàn AT chénróngdá fēixiǎoxíngxìbāofèiáibìngrénzhěnduànqiándetánmǒpiànzhōngzhēncèp53jīyīntūbiàn |
_version_ |
1716849435609137152 |