超臨界混合流體萃取銀杏葉的內酯及黃酮類

• Main Authors: Chiu, Kuo-Lung, 邱國隆
• Other Authors: 張傑明
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/65117255101863478491

碩士 === 國立中興大學 === 化學工程學系 === 88 === This study investigated the feasibility of extracting terpene lactones and flavonoids from Ginkgo leaves by using supercritical carbon dioxide, nitrous oxide, and R134a, respectively. A semi-continuous extraction and absorption process was employed to simultaneously collect and dissolve solutes precipitated from supercritical fluids. The extraction conditions were from 24.2 to 31.2MPa and from 333 to 393K. A RP-HPLC system coupled with RI and UV detectors were utilized to individually quantitate the levels of terpene lactones and flavonoid aglycones in the extracts. Our results demonstrated that three dry supercritical fluids, individually at the density of 0.844, 0.828 and 1.131 g/mL, were unable to extract terpene lactones and flavonoids. However, when supercritical fluid extraction with the addition of ethanol, the extracted amount of terpene lactones increased with the density of three supercritical fluids and the mole fraction of ethanol addition. Smaller particle size increased the amount of terpene lactones in SC--(90mol% CO2+10mol% EtOH) extraction and SC-(90mol% CO2+10mol% EtOH) was able to extract more terpene lactones than other two fluids. Finally, when 31.2MPa, 333K and 300L SC-CO2 extracted 90g dry leaves, suspended in 180mL EtOH, the yield of terpene lactones and flavonoids was 91% (i.e. 985μg/g) and 29% (i.e. 1342μg/g) of that extracted by EtOH Soxhlet extraction, respectively. It was found that a tablet, which raw material imported from Germany, contained the highest amounts of terpene lactones and flavonoids amoung four commercialized tablets.