Evaluation of Chitosan-Alginate-Hyaluronate Complexes Modified by RGD as Tissue Engineering Scaffolds for Cartilage Regeneration

• Main Authors: shu-chih hsieh, 謝淑枝
• Other Authors: shan-hui hsu
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/62418634510112791960

碩士 === 國立中興大學 === 化學系 === 88 === Abstract In this study, a series of natural biodegradable materials in the form of chitosan (C)-alginate (A)-hyalurante (H) complexes were evaluated as tissue engineering scaffolds. The ratio of C/A was 1:1 or 1:2. Hyaluronate was mixed in 2% of the C/A total volume. The complexes could be further modified by an RGD-containing peptide (R). Cytocompatibility tests of these complexes were conducted using immortalized rat chondrocytes (IRC) as well as primary articular chondrocytes harvested from animals. The formation of neocartilage was examined in rabbits after the scaffolds were implanted into the defected joints. The results from IRC and primary articular chondrocytes demonstrated that RGD enhanced cell attachment and spreading, but had no effect on proliferation at a later time. C1A1HR had better performance than the other complexes in cytocompatibility tests. Cells retained their spherical morphology on all C/A and C/A/H complexes. The 3-D in vitro evaluation included the cell number (DNA analysis), GAG content and collagen analysis. Results indicated that C1A1HR complex was the most appropriate for chondrocyte seeding. Scaffolds were implanted into the cartilage defects. After one month, partial repair has been observed in C1A1 and C1A1HR scaffolds. It was concluded that C1A1HR was a potential tissue engineering scaffold for cartilage regeneration.