Studies on the Blood Groups of Serum Proteins and Hemologlobin in Ostriches

• Main Authors: Shu-Lin Liu, 劉淑琳
• Other Authors: Mu-Chiou Huang
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/92329466109782540434

碩士 === 國立中興大學 === 畜產學系 === 88 === 英文摘要 Studies on the Blood Groups of Serum Proteins and Hemologlobin in Ostrich Shu-Lin Liu Abstract The purpose of this study is to investigate the blood protein patterns of blue neck, black neck and red neck ostriches. Through estimating differences of the allele frequency between various breeds, the study compared the varieties of hereditary forms between different breeds of ostriches. The study also compared the particular serum protein patterns of ostriches, emus and White Chinese geese. The results of Titan gel serum protein analysis showed: The level of serum protein of blue neck, black neck and red neck ostriches does not have significant differences (P>0.05). The percentages of albumin and α-globulin between male and female ostriches differ significantly (P<0.05). The pre-albumin and β-globulin of White Chinese geese significantly differ from ostriches and emus (P<0.05). The albumin and γ-globulin of ostriches and White Chinese geese differ significantly (P<0.05). The α-globulin of ostriches does not differ significantly from emus (P>0.05), but it is obviously higher than that of White Chinese geese (P<0.05). The pattern of lactate dehydrogenase in ostrich serum has five bands. The pattern of creatine phosphokinase in ostrich serum is CPK3 (MM) whose concentration is higher than CPK2 (MB) but does not have CPK1 (BB) bands. Ostrich, emu and White Chinese goose lactate dehydrogenase and creatine phosphokinase patterns are different. Using polyacrylamide gel electrophoresis to analyze the patterns of protein in ostrich serum and using cellulose acetate electrophoresis to analyze ostrich hemoglobin type, the results showed that the pre-albumin and albumin in blue neck, black neck and red neck ostrich serum have polymorphism while their transferrin and hemoglobin do not. The proportion of polymorphic loci of the three breeds of ostriches is the same (0.67). The result showed that the process of breeding was not strict, so the variety of heredity is significant. The number of effective loci of the three breeds of ostriches is higher than average (2.00±0.6). From this, it can be inferred that the allele of loci was randomly selected. The heterozygosity of pre-albumin in the three breeds of ostriches is higher than albumin (0.44~0.62 compared to 0.16~0.24). This showed that the variety of loci of the pre-albumin is great in ostriches. The mean of heterozygosity in the three breeds of ostriches is higher than average, and the cause might be that the ostriches are hybrids. The result matches the process of breeding ostriches.