Characteristics of Agrobacterium tumefaciens and Biological Control of Rose Crown Gall Disease in Taiwan

• Main Authors: Chia-Hsing Shen, 沈嘉興
• Other Authors: Shih-Tien Hsu
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/82329016002976215842

碩士 === 國立中興大學 === 植物病理學系 === 88 === During recent years, crown gall disease has been found on rose, Ficus spp. and aster in Taiwan. Thirty-nine bacterial strains including 37 strains isolated from the galls of rose collected in Changhua, Nantou, Yunlin and Taichung, one strain obtained from the galls of aster in Pingtung, and the Agrobacterium radiobacter K1206 strain from Australia were used in this study. All strains had been tested for their ability to induce crown galls on carrot slices, tomato seedlings and rose seedlings. These bacterial strains were also tested by PCR analysis with Agrobacterium tumefaciens specific primer VirD1. The results showed that 33 strains could induce galls. And all of these 33 strains except AA19 which was obtained from aster, could be amplified the specific DNA fragment in PCR analysis. Based on cultural, physiological and biochemical tests, bacterial strains from rose crown gall in Taiwan were separated into two clusters when subjected to numerical taxonomic analysis. The first cluster had the characteristics which were distinct from both biovar 1 and biovar 2, whereas the second cluster had the characteristics similar to biovar 1. Biolog GN System was also used in this study to identify bacterial strains from rose crown gall in Taiwan. Based on the pathogenicity test, physiological and biochemical testes and Biolog identification, the 32 bacterial strains from rose in Taiwan were identified as A. tumefaciens. The control of crown gall on rose cuttings by immersion in A. radiobacter K1026 bacterial suspension before planting was tested. The results showed highly significant disease control. Galls were absent or less on the roots of rose seedlings that had been dipped in a suspension of K1026, and planted in soil infested with A. tumefaciens. A. radiobacter K1026 also effectively protected the pruning wounds from infection by A. tumefaciens.