| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 88 === Summary
The germination percentage of "MESA659" lettuce seeds was 98 ﹪at 25 ℃. After seeds imbibed at 35 ℃ for 7 days, the seeds had 51 ﹪germination at 25 ℃. The seeds can not germination at 35 ℃. The seeds had 87.6 ﹪germination at 35 ℃ when seeds were primed by -1.1 Mpa PEG8000 for 3 days and 10 mM GA3+ 10 ppm Ethrel + 100μM BA treatments. Seeds were increased 2 ﹪germination and the mean days shorten 0.3 days after light treatment. The germination of artificially aged seeds were 68.9 ﹪at 25 ℃. Priming and hormone treatments could enhance 32 ﹪germination of artificially aged seeds at 35 ℃.
The amount of total peroxide and malondialdehyde were increased, the activity of superoxide dismutase, catalase, peroxidase, glutathione reductase, and glutathione-S-transferase were derceased. The amount of glutathione oxidized form, glutathione reduced form, and glutathione were derceased in artificially aged seeds and seeds imbibed at high temperature. Priming and hormone treatments could improve the antioxidation of seeds imbibed at high temperature. The activity of α-amylase, β-amylase, and the content of soluble carbohydrate of seeds imbibed at 35 ℃decrease 26 ﹪, 24 ﹪, and 15 ﹪, respectively. The amount of soluble protein of seeds imbibed at 35 ℃ decrease 15 ﹪compared to seeds at 25 ℃, moreover the protein amount of aged seeds decrease 39 ﹪. The amount of soluble protein, soluble carbohydrate, CO2,and ethylene were increased in primed seeds and seeds treated hormone at 25 ℃ and 35 ℃.
The amount of Mr. 66.2, 35.6, 32.4, and 27.2 KD protein increased on primed seeds imbibed at 25 ℃ and 35 ℃. Primed seeds imbibed at 35 ℃ appeared Mr. 33.8 KD and Mr. 31 KD.
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