Determination of morphine in urine by automatic sequential injection analysis atmospheric pressure chemical ionization and electrospray mass spectrometry
碩士 === 國立東華大學 === 化學研究所 === 88 === An automatic sequential injection analysis (SIA) method using immunoaffinity technique coupling with APCI and ESI mass spectrometry was developed for morphine determination in the urine. A polyclonal morphine antibody immobilized on the matrix of controlled porous...
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2000
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| Online Access: | http://ndltd.ncl.edu.tw/handle/87629664882458471060 |
| Summary: | 碩士 === 國立東華大學 === 化學研究所 === 88 === An automatic sequential injection analysis (SIA) method using immunoaffinity technique coupling with APCI and ESI mass spectrometry was developed for morphine determination in the urine. A polyclonal morphine antibody immobilized on the matrix of controlled porous glass (CPG) as reactor was mounted in SIA system to on-line affinity extraction the morphine in the urine sample during analysis. The deuterated analogue (morphine-D6, 100ppb) as internal standard was used for quantitative purpose. The 10 ppb and 50 ppb limit of detection (S/N＞3) were obtained in SIA-APCI-MS and SIA-ESI-MS, respectively. It shows at least six times sensitive than 300 ppb (the official cut-off of morphine in the urine). A good specificity was found that expect heroin and codeine have extremely slight interfering (no effect in normal analysis), most abused drugs including the morphine-glucuronide have no obviously interference. The time for one analysis is only 13 min and no significantly activity decline during 6 weeks testing shows a acceptable stability for measurements. The results obtained from the real urine sample measuring by GC-MS and this method shows good accordance.
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