The mechanism of dengue virus infection of human monocyte

• Main Authors: Tsai, Fu-Ming., 蔡福明
• Other Authors: Lai
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/72974150643731171621

碩士 === 國防醫學院 === 微生物及免疫學研究所 === 88 === Dengue virus is an arthropod-borne human pathogen that represents a serious public health threat. The clinical manifestations of dengue virus infection range in severity from a simple febrile illness to a hemorrhagic fever and a potentially fatal hemorrhagic shock syndrome. Dengue virus gains entry to some cell types by an immune adherence bridging phenomenon which is dependent on the presence of nonneutralizing antibodies. However, this mechanism cannot explain primary infections in patients without antibody to dengue virus and the ability of dengue virus to infect non-phagocytic cell types that do not express Fc receptors. In this study, we treated dengue virus with various GAGs to observe if GAGs can inhibit virus infection. The result indicated that just only heparin could inhibit virus infection. This suggests that heparan sulfate may be an important factor for the penetration of virus inot the host cells. On other hand, we found that the expressing of CD14 couldn’t increase cell’s susceptibility to dengue virus infection. The possible explanation is that the activation of cell by vitamin D3 made cell more resistant to virus infection. Besides, we found that the dengue virus infected cells released more cytokine, TNF-alpha. The phenomenon is similar to those patients with DHF and DSS. To reveal why dengue virus can induce the serious syndrome in the secondary infection to human monocyte need further study.