| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系 === 88 === The objectives of this study were to investigate the antimutagenic activity of 50% alcohol extracted Anoectochilus formosanus Hayata, Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu., Chrysanthemum indicum L., Glossogyne tenuifolia (Labill) Cass, Lycium chinense Mill, Rhinacanthus nasutus (L.) Kurz, Boehmeria nivea (L.) Gaud. var. nivea, Agastache rugosa (Fisch et Mey) O. Kuntze, Ganoderma lucidum (Leyss. ex. Fr.) Karst, and water extracted Anoectochilus formosanus Hayata and Morus australis Poir.
The studies were investigated by Ames test using Salmonella typhimurium TA98、TA100、TA1535 and immediate mutagen of NQNO (without S9 mix) and indirect mutagen of B[a]P (add S9 mix) to these ten plants. Five different dosage were used, which were 0.5, 1.0, 2.0, 2.5, 5.0 mg/plate. The toxicity were found at Ganoderma lucidum (Leyss. ex. Fr.) Karst, Agastache rugosa (Fisch et Mey) O. Kuntze, Chrysanthemum indicum L., Glossogyne tenuifolia (Labill) Cass, Lycium chinense Mill, Rhinacanthus nasutus (L.) Kurz, Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu., Anoectochilus formosanus Hayata (water extracted), Morus australis Poir without S9 mix, Glossogyne tenuifolia (Labill) Cass, Anoectochilus formosanus Hayata (alcohol and water extracted) with S9 mix for TA 98, and Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu. without S9 mix, Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu. and Lycium chinense Mill with S9 mix at the dosage of 5 mg/plate. But at the reduced dosage level of 2.5 mg/plate, the toxicity was not found. The mutagenicity was not found at every dosage tested for all the samples.
In the antimutagenic activity, effect against B[a]P toward TA 1535 were found for all samples, others were dependent on tested samples. Anoectochilus formosanus Hayata (alcohol extracted) had to effect against NQNO toward TA 98. Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu. showed effect gainst NQNO toward TA 98. Chrysanthemum indicum L. exhibited effect to against NQNO toward TA 100. Glossogyne tenuifolia (Labill) Cass reveled effect against NQNO toward TA 1535. Morus australis Poir had effect to against B[a]P toward TA 98 and NQNO toward TA 100.Lycium chinense Mill showed effect gainst B[a]P toward TA 98. Rhinacanthus nasutus (L.) Kurz had effect against NQNO toward TA 98. Boehmeria nivea (L.) Gaud. var. nivea had effect against NQNO toward TA 98 and TA 100. Agastache rugosa (Fisch et Mey) O. Kuntze had effect against NQNO toward TA 1535. Ganoderma lucidum (Leyss. ex. Fr.) Karst had effect against NQNO toward TA 98 and TA 100. Significantly antimutagenic activities were found for Anoectochilus formosanus Hayata (extracted alcohol), Vitis thunbergii Sieb. & Zucc. var. taiwaniana Lu., Rhinacanthus nasutus (L.) Kurz, Boehmeria nivea (L.) Gaud. var. nivea and Ganoderma lucidum (Leyss. ex. Fr.) Karst against NONO, and Morus australis Poir and Lycium chinense Mill against B[a]P toward TA 98, Chrysanthemum indicum L., Morus australis Poir, Boehmeria nivea (L.) Gaud. var. nivea and Ganoderma lucidum (Leyss. ex. Fr.) Karst against NONO, but showed no effect against B[a]P toward TA 100, Glossogyne tenuifolia (Labill) Cass and Agastache rugosa (Fisch et Mey) O. Kuntze against NONO.
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