| Summary: | 碩士 === 國立臺灣大學 === 森林學研究所 === 88 === Results of mature embryo culture of Tsuga chinensis (Franchet) Pritz. ex Diels var. formosana (Hayata) Li ＆ Keng were as follows: mature seeds were first treated with running water for 48 hours, then soaked in 70% ethanol solution for 2 minutes. Soaked in 5%NaOCl (supplemented with 1% (v/v) Tween 20) and treated with ultrasonic shaker for 20 minutes. The medium containing 0.1% (v/v) EM-X could promote germination ratio of mature embryos. Light brown and soft calli were induced after mature embryos were cultured on MS medium containing 1 ppm BA and 2 ppm 2,4-D under dark condition. Mature embryos grew better in MS medium containing 0.1ppm TDZ under light condition. Adventitious buds formed on SH medium containing BA or TDZ or BA with 2,4-D. Under dark condition, white and compact calli were induced in SH medium containing BA and 2,4-D. After the adventitious buds were transferred to 1/2 strength SH medium containing 0.1%(w/v) activated charcoal, and then transferred to 1/2 SH medium with out activated charcoal alternatively, adventitious buds could grow to plantlets.
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