| Summary: | 碩士 === 國立臺灣大學 === 農業化學研究所 === 88 === Ganoderma lucidum is a Chinese herb, has been widely used in Asian countries such as China, Japan and Korea for a long time. Several studies reported that Ganoderma lucidum could be applied for treatment in diseases such as hypertension, hyperlycemia and allergic diseases. Furthemore, Ganoderma lucidum has been reported to exert anti-tumor activity and activation of natural killer cells (NK-cells). In this study, BALB/c mice were fed with different dose of Ganoderma tsuga YK-01 or YK-07 by tube feeding daily for 2 months''''. After 2 months experimental treatment, BALB/c mice were sacrified for NK-cell activity, phagocytic activity and cytokine analysis.
The results of non-specific immune response showed that tube feeding of Ganoderma tsuga did not affect the growth curve and food efficiency of the mice. IL-1b and IL-6 secreted by macrophage of mice fed low does of Ganoderma tsuga YK-01 were lower than those of control group. IL-1b and IL-6 secreted by macrophage of mice fed Ganoderma tsuga YK-07 were higher than control group. In addition, the natural killer cell activity in mice was significantly higher compared to that of control mice. Cytokine profiles demonstrated that cytokines such as IL-2 and TNF-a produced by spleen cells were higher in high does group. In contract, type 2 related cytokines such as IL-4 levels were lower in mice of high does group compared to those of control mice. Furthermore, immunoglobulin G, M and A levels were all significantly higher in mice fed Ganoderma tsuga.
The results of specific immune response showed that tube feeding of Ganoderma tsuga did not affect the growth curve and food efficiency of the mice. The natural killer cell activity in mice was also higher compared to that of control mice. Cytokine profiles demonstrated that cytokines such as IL-2 produced by spleen cells were lower than control group. Type 2 related cytokines such as IL-4 and IL-5 levels were also lower in mice of high does group compared to those of control mice. Furthermore, immunoglobulin G and A levels were all significantly lower in mice fed Ganoderma tsuga.
Supplementation of Ganoderma tsuga did influence many immune responses in vivo. The activity analysis of NK-cells from peritoneal cells were the best evaluated method of immuno-modulation function.
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