| Summary: | 碩士 === 中國文化大學 === 生活應用科學研究所 === 88 === According to the study on dietary fiber intake for Taiwanese, only half amount of daily recommended allowance had been taken. Epidemiological investigation also demonstrated that dietary fiber plays an important role in the prevention of colon cancer. Since the gut-associated lymphoid tissue（GALT）-peyer’s patches of the epithelium mucosal cells is the primary defense system of the intestine against the foreign antigen, we wonder if dietary fiber affects the development of GALT and then further affects the intestinal immunity.
Three-to-four-week-old Wistar rats were divided into ten groups. In addition to the control group fed without fiber，the treatment groups were fed with cellulose, wheat bran, and rice bran for 8 weeks with various doses（w/w：5﹪, 10﹪, and 20﹪） respectively .First of all, we drew the blood from heart artery for analyzing serum immunoglobulins IgA、IgG、IgM by method of enzyme-linked immunosorbent assay（ELISA）. Secondly, we collected peyer’s patches（PP.）closed to ileum-cecum value to make paraffin embedding and then observed the development of PP by staining with haematoxylin and eosin . We also performed frozen section of immunohistochemistry to exam the distribution of B lymphocytes and T lymphocytes in PP.
The results showed that there were more follicles in PP for cellulose, rice bran and wheat bran groups than those for control group. We also noticed that no significant inferfollicle areas and villi for the groups fed with different dietary fiber. Frozen section of immunohistochemistry have showed that there were more B cell by staining in the dome area of peyer’s patches for cellulose, rice bran and wheat bran groups than those for control group.
The treatment groups exhibited lesser immunoglobulin G and M compared to control group. It is possible that lower levels of immunoglobulin secreted from lymph nodes were caused by lesser antigen stimulation. It is worthy to be further studied.
|
|---|
