（一） Effect of dextromethorphan in preventing the morphine withdrawal syndrome in the neonatal rat （二） Effect of chronic treatment of morphine on the expression of the NMDA receptor in the developing rat brain. （三） Effect of chronic treatment of morphine

• Main Authors: Tsan-Lin Hu, 胡璨麟
• Other Authors: Geng-chang Yeh
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/26429791317271832839

碩士 === 台北醫學院 === 細胞及分子生物研究所 === 88 === It has been reported that infants born to morphine or heroin addicted women have high incidence of neonatal abstinence syndrome and abnormal neuropsychological performance. Preventing of neonatal abstinence syndrome and defining the neurotoxicity of morphine in the developing brain are important works. Our previous studies found that dam rat injection of dextromethorphan can attenuate the naloxone induced withdrawal syndrome. We also found that ontogenic expression of the NMDA receptor in this rats is different to that of control rat by lacking an overshooting of NMDAreceptor density on PND14. It is possible that this transient change is due to decrease in the subtype of the NMDA receptor or a decrease in the numbers of the synapse that containing the NMDA receptor. We used the same morphine treatment animal modal to further explore（1）whether prenatal treatment with dextromethorphan on the dam rats could prevent the morphine withdrawal syndrome in their off springs.（2）whether morphine treatment could induce a change in the NMDA receptor subunits in the developing rat brain（3）whether morphine treatment will change the the expression and degradation of the spectrin in the developing rat brain. We found that pre-treatment with dextromethorphan on the dam rats can prevent morphine withdrawal syndrome in the neonatal rat born to morphine treated dam rat. The expression of NMDA receptor subunit in the cortex, hippocampus and striatum of morphine group rats is decreased at PND14 as compare to that of control group. In the cortex, NR1A, NR2A, NR2B, NR2C are decreased on PND7 and PND14； in the hippocampus, NR2A, NR2B, NR2C are decreased on the PND7 ； in the striatum, NR1A, NR2A, NR2B, NR2C are decreased on PND7 and PND14. We also found that the expression of spectrin in the rat brain of morphine rats is decreased as compare to that of control group. In cortex α-spectrin decreased on PND7, PND14, PND30, andβ-spectrin decreased on PND7, PND14. In the hippocampus the α-spectrin decreased on PND7 andβ-spectrin on PND7. In the striatum the α-spectrin decreased on PND7 andβ-spectrin decrease on PND7, PND14, PND60.