Treatment and Immunity Modulation of the L5178Y Murine tumor- dormant state byBu-Zhong-Yi-Qi-Tan

• Main Authors: Ching-Yuan Lai, 賴慶元
• Other Authors: Chih-Hsiung Wu
• Format: Others
• Language: en_US
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/21645996135197637047

碩士 === 台北醫學院 === 醫學研究所 === 88 === Abstract Herbs are very popular in clinical especially in the usage of preventing the recurrence of cancer in Taiwan. The benefit of herbs was low side effects, low price and easy to used compared to current immune therapy but the question come if the herbs really have an effect of reducing cancer, lack of scientific research was a common answer. So it is important for us to test the usage of herbs in increasing our immune system and reduce cancer in a animal test model. The paper uses DBA/2 mice by injecting L5178Y Cancer cell epidermally. After inducing the formation of the tumor , we remove the tumor form the injection site and then inject L5178Y into the abdominal cavity of the mice. By creating a balance amount between the tumor cell and the mice’s immune system, to be cancer dormancy. Herbs (Bu-zhong-yi-Qi-Tan) was fed to the mice for over 7 days. And then some amount of the ascites (partial peritoneal larvage, PPL) was taken from the abdominal cavity of the mice. Then analysis the PPL to test the amount of the L5178Y tumor cell count and the difference of CD-4 and CD-8 and the difference of the NK cell and the amount of Macrophage by using Flow Cytometry. The result was the amount of L5178Y decrease and the amount of CD-4 increases, and no difference was found between the control group which was fed only with normal saline. The result by using animal test model has conclude that herbs was effective in the treatment of cancer dormancy in increase the T-cell immune system and reduce the cancer cell level. So the Bu-zhong-Yi-Qi-Tan was effective in a immune therapy. The animal test model could play a way to check the immunity system.