Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA
碩士 === 國立陽明大學 === 微生物暨免疫學研究所 === 88 === The double-spliced RNA (dsp-RNA) is a naturally produced RNA by HBV (10). From previous studies, it has been demonstrated that the double-spliced RNA, pCMV/DS/3081, played an inhibitory role in the expression of HBV genes and this inhibition was regulated at t...
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ndltd-TW-088YM0003800232016-01-29T04:19:38Z http://ndltd.ncl.edu.tw/handle/27850612380723299090 Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA B型肝炎病毒雙剪接核醣核酸抑制作用之機制研究 Ren-Kou Lin 林仁國 碩士 國立陽明大學 微生物暨免疫學研究所 88 The double-spliced RNA (dsp-RNA) is a naturally produced RNA by HBV (10). From previous studies, it has been demonstrated that the double-spliced RNA, pCMV/DS/3081, played an inhibitory role in the expression of HBV genes and this inhibition was regulated at the level of the transcription initiation (29). In this thesis, we would like to study further on the inhibitory role of the double-spliced RNA in HBV. The results are as follows: First, it is demonstrated that the 5’ end of transcript from pCMV/DS/3081, in which 162 nucleotides was transcribed from the putative transcription start site of CMV promoter, is not essential for the inhibitory effect. Also the inhibitory effect of the dsp-RNA could be observed by 24 hours after transfection. Second, five deletion clones were used to map the important regions for the inhibition. The data indicate that the 5’ region of the double-spliced RNA is crucial to the inhibitory activity. Finally, luciferase gene that was controlled either by single transcription element (TATA box) or complex up-regulatory cis-elements (authentic cytomealovirus immediate early promoter). We found that the dsp-RNA exhibited similar inhibitory activity in both systems. This results suggest that dsp-RNA inhibits the gene expression through its interaction with common trancriptional factors of TATA box. Chungming Chang 張仲明 2000 學位論文 ; thesis 50 zh-TW |
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碩士 === 國立陽明大學 === 微生物暨免疫學研究所 === 88 === The double-spliced RNA (dsp-RNA) is a naturally produced RNA by HBV (10). From previous studies, it has been demonstrated that the double-spliced RNA, pCMV/DS/3081, played an inhibitory role in the expression of HBV genes and this inhibition was regulated at the level of the transcription initiation (29). In this thesis, we would like to study further on the inhibitory role of the double-spliced RNA in HBV. The results are as follows: First, it is demonstrated that the 5’ end of transcript from pCMV/DS/3081, in which 162 nucleotides was transcribed from the putative transcription start site of CMV promoter, is not essential for the inhibitory effect. Also the inhibitory effect of the dsp-RNA could be observed by 24 hours after transfection. Second, five deletion clones were used to map the important regions for the inhibition. The data indicate that the 5’ region of the double-spliced RNA is crucial to the inhibitory activity. Finally, luciferase gene that was controlled either by single transcription element (TATA box) or complex up-regulatory cis-elements (authentic cytomealovirus immediate early promoter). We found that the dsp-RNA exhibited similar inhibitory activity in both systems. This results suggest that dsp-RNA inhibits the gene expression through its interaction with common trancriptional factors of TATA box.
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author2 |
Chungming Chang |
author_facet |
Chungming Chang Ren-Kou Lin 林仁國 |
author |
Ren-Kou Lin 林仁國 |
spellingShingle |
Ren-Kou Lin 林仁國 Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
author_sort |
Ren-Kou Lin |
title |
Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
title_short |
Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
title_full |
Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
title_fullStr |
Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
title_full_unstemmed |
Study on the Inhibitory Mechanism of a Hepatitis B Virus Double-Spliced RNA |
title_sort |
study on the inhibitory mechanism of a hepatitis b virus double-spliced rna |
publishDate |
2000 |
url |
http://ndltd.ncl.edu.tw/handle/27850612380723299090 |
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