The study of relationship between hypoxanthine-guanine phosphoribosyltransferase activity and gout or uric acid

• Main Authors: Shiu-Min Chen, 陳秀敏
• Other Authors: Ying-Chin Ko
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/02530974026597526444

碩士 === 高雄醫學大學 === 公共衛生學研究所 === 89 === Abstract There are two aboriginal tribes (Bunun, Paiwan) and one non-aboriginal tribe (Fukien-Taiwan) included as the study population. The subjects were used to explore the relationship between the activity of hypoxanthine-guanine phosphoribosyltransferase (HPRT) and gout disease or uric acid level. Comparison the difference of HPRT activity between aborigines and non-aborigines and the related factors are the study major aims. A total of 172 samples included in this study. A questionnaire was completed by the subjects to obtain the social demographic data, and a whole blood was drawn to perform the biochemical and HPRT activity analysis. A screening method for detection of HPRT activity in peripheral blood mononuclear cells (PBMCs) was used by high-performance liquid chromatography (HPLC). The results showed that： (1)The mean HPRT activities of subjects with gout and non-gout were 6.76±3.69 nmol/106 cell/h and 7.36±3.50 nmol/106 cell/h, respectively, and they did not make difference in statistics. (2)The mean HPRT activities of subjects aborigines and non-aborigines were 6.90±3.45 nmol/106 cell/h and 7.32±3.85 nmol/106 cell/h, respectively, and they did not different. (3)The mean HPRT activity of women with gout was 5.48±2.89 nmol/106 cell/h that had significant difference to femeal contol was 7.94±3.78 nmol/106 cell/h. (4)The related factors of enzyme activity were amount smoking, history of gout,and triglyceride. The study’s conclusion was the relationship of HPRT activity and gout or uric acid level had not to arrive at significant, and the reason was maybe we could not avoide counfounding of treatment with gouty drug. Key word: HPRT, HPLC, GOUT, URIC ACID