| Summary: | 碩士 === 國立中央大學 === 生命科學研究所 === 89 === Arsenical compounds are widely distributed in the water, soil
and air. Epidemiological evidences have revealed that arsenic
exposure is associated with increased incidences of a variety of
diseases, such as Blackfoot Disease, and skin, liver, bladder and lung
cancers. Previous studies in our laboratory have shown that
treatment of HeLa S3 cells with 5 μM arsenite leads to about 35% of
the cells arrested in the mitotic metaphase. Treatment of
arsenite-arrested mitotic metaphase cells with staurosporine (protein
kinase inhibitor) enforces them exiting from mitosis. In this study,
we try to observe changes of ultrastructure and investigate protein
modifications by staurosporine on arsenite-arrested mitotic cells.
Our results show that treatment of arsenite-arrested mitotic cells
with staurosporine leads to dissolution of chromosome and
elongated and condensed spindle, reformation of nuclear envelope
and generation of multiple nuclei. In addition, proteins modified by
staurosporine treatment on arsenite-arrested mitotic cells are also
observed by two-dimentional gel electrophoretic analysis. However,
these proteins should be further identified and characterized by
MALDI-TOF or 2D-Western techniques.
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