Comparison of the pathological changes in dendritic cell and mosquito C6/36 after dengue virus type 2 infections.

• Main Authors: Chien-Ting Lin, 林建廷
• Other Authors: Jaang-Jiun Wang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/82705944761458360727

碩士 === 國防醫學院 === 生物及解剖學研究所 === 89 === Human dendritic cells (DC) derived from bone marrow, are one of the most potent antigen-presenting cells (APC) essential for the induction of immune responses. The interaction between DC and dengue virus (DV) has been reported in many aspects. However, the cytopathological changes induced by DV were unclear yet. The mosquito cell line C6/36 cells could be infected by dengue virus and replicated dengue virus well. Compared with dendiritic cells, C6/36 cells did not have the ability of antigen presenting cells. So we postulated that there must be some different cytopathological responses between C6/36 cells and DC. In this study, a comparasion was made between the well-known mosquito cell line C6/36 cells and DC after dengue virus type 2 infections. After 24 and 48 hours of viral infections of both DC and C6/36 cells, NS1 was shown in the cytoplasm, but at different localizations. Hypertrophy of rER, cystic vesicles and swelling of mitochondria were also observed in both cells. The Den-2 particles were found in the cellular vesicles, dilated lumens of rough endoplasmic reticulum (rER), and in the vacuoles after 24 and 48 hrs infections, but the particles in C6/36 were almost found in large vacuoles. Immunogold stain showed that virus protein E was presents in the cytoplasm, rER and around the nucleus. Apoptosis appeared in dendritic cells after infected for 24 hrs, but not in C6/36. These observations provided morphological evidences that different cytopathological responses in DC and C6/36 were induced by dengue virus type 2 infections. Further investigation for the significance of these structural changes is necessary.