| Summary: | 碩士 === 國立臺灣師範大學 === 生物研究所 === 89 === Abstract
It has been demonstrated that baicalin extracted from the root of Huangquin, a Chinese herb medicine has potential antioxidant and anti-inflammatory effect. Many studies have indicated that overproduction of nitric oxide (NO), from inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2) from cyclooxygenase-2 (COX-2), free radical, and tumor necrosis factor-a (TNF-a) is involved in the pathogenesis of septic shock. In this study, we evaluated the effect of baicalin on these mediators and iNOS as well as COX-2 expression in lipopolysaccharide (LPS)-treated RAW264.7 macrophage.
Our results demonstrated that baicalin ( 10~50 mM ) concentration-dependently inhibited LPS-induced NO、TNF-a and PGE2 production in LPS-treated RAW264.7 macrophage without an appreciable cytotoxic effect. Furthermore, baicalin inhibited the expression of LPS-induced iNOS and COX-2 proteins and mRNAs. These results indicated that the inhibition of TNF-a production by baicalin maybe contribute to the suppression of iNOS and COX-2 expressions. Thus, baicalin may be beneficial for treatment of endotoxaemia. Besides, treatment of the RAW264.7 macrophage with 50 mM baicalin decreased the S phase and G2/M phase ratio about 15% in the cell cycle. Thus, baicalin may be also a potential therapeutic drug for tumor therapy.
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