| Summary: | 碩士 === 國立臺灣大學 === 園藝學研究所 === 89 === ound hydrophobic lignans (sesamolin, pinoresinol, sesaminol, sesamin) and hydrophilic lignan glycosides. Most of the sesame oil is produced by roasting and pressing. After high temperature roasting, the sesame meal appeared bitter taste and its protein was highly denatured. Therefore, the sesame meal is generally used as feeds or fertilizers. The purpose of this study was to extract the sesame meal in supercritical carbon dioxide (SC-CO2) and different solvents. The extracted after various solvents treatment were analyzed to determine the optimum processing condition.We hope we can increase the amount of functional components in sesame meal to enhance the utilization of sesame meal.
The sesame meal was extracted with five solvents (n-hexane, ethyl acetate, ethanol, methanol and water). Ethanol extracts exhibited strong DPPH( 2,2-diphenyl- 1-picrylhydrazyl) free radical scavenging effect. When various concentration ethanol extract, 80% ethanol crude extracts showed good antioxidative activity toward DPPH free radical scavenging effect and inhibition of lipide oxidation(88.51%, 73.25%). Crude extracts (80% ethanol) have significantly content on brown index and sesaminol triglucoside content.
To investigate the effect of an entrainer on SC-CO2. Using ethanol as an entrainer on SC-CO2 than added to the sample would give strong DPPH free radical scavenging effect. At constant temperature 50 to 70℃, decreased the amount SC-CO2 (25-50NL), raising temperature increased sesamol contents. At 70℃/fraction 2 have high content (0.452 mg/g crude extract). Under the operation temperature from 50℃ to 70℃, the DPPH free radical scavenging effect were decreased with increasing temperature. At 50℃/fraction 4 showed better DPPH free radical scavenging effect(77.43%), crude extracts have significantly content on brown index and total phenolics content. At constant temperature (50℃), pressure (4000psi) and the amount of ethanol (10mol%) , fraction 4 showed the largest sesamol contents (0.134 mg/crude extract) and total phenolics content(0.229 mg/crude extract). The crude extracts showed good antioxidative activity toward DPPH free radical scavenging effect (77.43%) and inhibition of lipide oxidation(77.73%).The incresment of antioxidative activity of crude extract of lignan glycosides was probably due to the total phenolics contents and maillard reaction products formed during roasting process.
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