| Summary: | 碩士 === 國立臺灣大學 === 電機工程學研究所 === 89 === The object of current study is to make use of the process of silicon semiconductor and the technique of MEMS to realize the DNA chip for micro electrophoresis. By the microchannel on silicon, we just need a small amount of reagent to be measured. We refer electrochemical methods and join our ideas to bring up some mathematic models to explain the behavior of these reagents in different conditions. With these results, we can distinguish some charged ions with different molecular weight. Therefore, it’s able to apply the method on DNA, and to distinguish different DNA by the chip.
This study has developed some simple different Si etchant for deep etching. And successfully, the Si etchant can make the microchannel be done. Furthermore, the current and voltage of these reagents can be measured precisely by high resolution measurement system. Combining with the electrical circuit method and the concept of physics and chemistry can build up mathematic model. Then,we simulate the model by computer programs.
After simulation by computer programs and the repeated experiments, it is feasible to distinguish different DNA molecule by the electrophoresis chip. It should be beneficial for the advance of biomedical diagnostic technology.
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