The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis
碩士 === 國立臺灣科技大學 === 化學工程系 === 89 === Optical active phenylglycine (PG) is important in medical, agriculture and food industries. The stereoselectivity of enzyme is employed for the hydrolysis of DL-benzylcarbonyl-PG ethyl ester. PG is insoluble in non-polar solvent, amino group of PG is...
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2001
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ndltd-TW-089NTUST3420162016-07-04T04:17:17Z http://ndltd.ncl.edu.tw/handle/92768625708912925693 The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis 以酵素進行苯基甘氨酸乙基酯水解光學分割之研究 Meng Yi Chen 陳孟儀 碩士 國立臺灣科技大學 化學工程系 89 Optical active phenylglycine (PG) is important in medical, agriculture and food industries. The stereoselectivity of enzyme is employed for the hydrolysis of DL-benzylcarbonyl-PG ethyl ester. PG is insoluble in non-polar solvent, amino group of PG is protected by benzylcarbonyl group for increasing the solubility of PG in solvent. The effects of reaction conditions on the reaction rate and enantionselectivity of the hydrolysis of DL-benzylcarbonyl-PG ethyl ester were investigated. When 100 mg Rhizopus delemar lipase was used as the catalyst , with hexane as the solvent and at a reaction temperature of 37℃, the conversion is 47.9 % after 12 h with enantioexcess (ees) and enantioselectivity (E) of 88.9 % and 170.6, respectively. If the lipase is immobilized, the same conversion can be attained within 6 h with E value of ca. 100 which is independent of the amount of immobilized enzyme. The hydrolysis of DL-benzylcarbonyl-PG ethyl ester can be described by Michaelis-Menten kinetics with Vmax = 0.128μmol ester/h mg enzyme and Km = 0.43 mM. 朱義旭 2001 學位論文 ; thesis 72 zh-TW |
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碩士 === 國立臺灣科技大學 === 化學工程系 === 89 === Optical active phenylglycine (PG) is important in medical, agriculture and food industries. The stereoselectivity of enzyme is employed for the hydrolysis of DL-benzylcarbonyl-PG ethyl ester. PG is insoluble in non-polar solvent, amino group of PG is protected by benzylcarbonyl group for increasing the solubility of PG in solvent. The effects of reaction conditions on the reaction rate and enantionselectivity of the hydrolysis of DL-benzylcarbonyl-PG ethyl ester were investigated.
When 100 mg Rhizopus delemar lipase was used as the catalyst , with hexane as the solvent and at a reaction temperature of 37℃, the conversion is 47.9 % after 12 h with enantioexcess (ees) and enantioselectivity (E) of 88.9 % and 170.6, respectively. If the lipase is immobilized, the same conversion can be attained within 6 h with E value of ca. 100 which is independent of the amount of immobilized enzyme. The hydrolysis of DL-benzylcarbonyl-PG ethyl ester can be described by Michaelis-Menten kinetics with Vmax = 0.128μmol ester/h mg enzyme and Km = 0.43 mM.
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author2 |
朱義旭 |
author_facet |
朱義旭 Meng Yi Chen 陳孟儀 |
author |
Meng Yi Chen 陳孟儀 |
spellingShingle |
Meng Yi Chen 陳孟儀 The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
author_sort |
Meng Yi Chen |
title |
The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
title_short |
The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
title_full |
The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
title_fullStr |
The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
title_full_unstemmed |
The study of DL-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
title_sort |
study of dl-phenylglycine ethyl ester resolution by enzyme catalyzed hydrolysis |
publishDate |
2001 |
url |
http://ndltd.ncl.edu.tw/handle/92768625708912925693 |
work_keys_str_mv |
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