Effects of C60 derivatives on learning and memory ability in senescence accelerated mice

• Main Authors: In-Lu Chen, 陳英茹
• Other Authors: Ming-Fu Wang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/57797086196779063957

碩士 === 靜宜大學 === 食品營養學系 === 89 === Water-soluble fullerence ( FC4S ) have been showed to against oxidative stress and could prevent neuron death effectively. The main purpose of this experiment was to evaluate the effects of different doses of FC4S on the learning and memory abilities in SAMP8 mice. 1-month-old SAMP8 mice were divided into control and three experimental groups, and injected 0.9% saline, 0.5mg/kgBw FC4S, 5mg/kgBw FC4S and 15mg/kgBw FC4S respectively by intraperitoneal for 36weeks. Food intake, body weight, and grading score were measured . The learning and memory abilities were evaluated by single-trial passive avoidance and active shuttle avoidance tests.The biochemical parameters, total antioxidant equivalent of serum and liver were analyzed after sacrificed, and the pathological examination of brains were also done. The result shows the food intake and body weight of three experimental groups were less than the control group , especially the group injected with 15mg/kgBw FC4S (P＜0.05). The grading score of the control group was higher than the experimental groups(P＜0.05). Single-trial passive avoidance test showed the experimental groups were significantly better than control group in the 24th, 48th, 72th hours and 7th day (P＜0.05). In the active shuttle avoidance test, the failure numbers of control group was obviously higher than the experimental groups in both male and female, indicate the learning and memory abilities of control group was worse. Total oxidant equivalences of serum and liver were higher in experimental groups, and 15g/kgBw FC4S group had the highest values(P<0.05). The groups injected FC4S had lower serum Alb, TG and glucose and could significantly reduce the accumulation of lipofuscin and spongy degeneration of brain when compared with control group. In summary, we conclude that the supply of FC4S may improve the learning and memory abilities and retard the aging process.