Using Salicyate Adducts as an Oxidative Stress Maker by GC/MS and Evaluate Hydroxyl Radicals Scavenging Effect of Polyphenols

• Main Authors: Ming-Yu Yang, 楊明玉
• Other Authors: Shiow-Yunn Sheu
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/40270312646595559672

碩士 === 台北醫學院 === 藥學研究所 === 89 === All aerobic organisms produced superoxide anion and hydrogen peroxide via respiratory burst. In the presence of chelated iron, these species are converted to hydroxyl radicals through a Fenton reaction . The hydroxyl radical is an extremely reactive oxidizing radical that will react with most biomolecules at diffusion-controlled rates (107-1010 M-1S-1), and cause membrane lipid peroxidation, protein aggregation, and DNA hydroxylation, thereby disrupting cellular functions and integrity. Because hydroxyl radical has a very short half-life and high reactivity, its measurement is very difficult. The trapping method with potential for specifically identifying hydroxyl radicals is aromatic hydroxylation. The method of salicylate produced hydroxylation adducts (2,3-DHBA and 2,5-DHBA) with hydroxyl radicals, was large used to assessment of the scavenging activity of hydroxyl radical both in vitro and in vivo. In vitro study, the polyphenols and others scavenged hydroxyl radicals generated by Fenton reaction. Free hydroxyl radicals were trapped by salicylate and adducts was detected by GC/MS. The scavenging activity of polyphenols decrease in the order: quercetin (55.8%) ， rutin (54.7%)， trolox (51.1%)， thiourea (50.1%)， myricetin (48.1%)， luteolin (45.7%)，(-)-epigallocatechin (41.9%)，(-)-epicatechin (41.7%)， morin (41.3%)，(+)-catechin (40.6%)，(+)-gallocatechin (38.8%)，kaempferol (38.1%)，wogonin (31.8%)，liquiritin (30.9%)，bacailein (30.2%)，DMSO (30.2%)，capillarisn (27.3%)，genistein (26.9%)，probucol (26.9%)，β-carotene (26.6%)，ascorbic acid (-)，d-α-tocopherol (-)。 In vivo study, the change of the amount of 2,3-DHBA and 2,5-DHBA were observed when the rabbits were treated with IP of salicylate and induced by 1,1,2,2-tetrachloroethane to inhibit cytochrom P450.