Cultivation and application of petroleum substances degradation bacteria

• Main Authors: Feng-Ming Wu, 吳俸銘
• Other Authors: Prof. Hung-Tuan Fang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/18991477868417181875

碩士 === 國立雲林科技大學 === 環境與安全工程系碩士班 === 89 === Petroleum hydrocarbons contain numerous substances including aliphatics, aromatics and resins can cause variability、carcinogenic and restrain growth of organism. In wastewater treatment system of refining sometimes it occurs shock-loading, and causes biotreatment systems running abnormal. We can add the bioaugmentation agents to solve the problem. This study isolated the indigenous hydrocarbon degrading bacteria strains, and investigated their optimal growth condition. And cultivate these bacteria in the fermentor and produce bioaugmentation agents. The bioaugmentation agent products can use to treat wastewater of petroleum industry and bioremediation of contamination region by spill oil. Three petroleum degradation bacteria were isolated from Taiwan nature environment, these bacteria were identified as diesel oil utilizer Pseudomonas aeruginosa DS-4, kerosene utilizer Burkhol cepacia KS-1 and phenol utilizer Agrob radiobacter PH-2. The optimal cultivation condition of DS-4 strain were determined: 0.5 % (v/v) diesel oil as carbon source, 500 mg/L (NH2)2CO+(NH4)2SO4 as nitrogen source, 500 mg/L KH2PO4 as phosphorus source, the optimal surfactant as Tween 40, pH 7 and 30℃. The optimal cultivation condition of KS-1 strain were determined: 1.0 % (v/v) kerosene as carbon source, 750 mg/L (NH2)2CO+(NH4)2SO4 as nitrogen source, 1000 mg/L KH2PO4 as phosphorus source, the optimal surfactant as Tween 40, pH 7 and 30℃. The optimal cultivation condition of PH-2 strain were determined: 500 mg/L phenol as carbon source, 500 mg/L (NH2)2CO+(NH4)2SO4 as nitrogen source, 1000 mg/L KH2PO4 as phosphorus source, pH 7 and 30℃. Petroleum degradation bacteria were cultivated in the fermentor under optimal condition. DS-4 strain cultivated with diesel oil as sole carbon source, it’s maximum specific growth rate μmax＝0.51 hr-1, saturated constant Ks＝1.21 g/L and saturated inhibited constant Ki＝1.96 g/L. 78 hours fermentation get maximum cell concentration 11 g/L. KS-1 strain cultivated with kerosene as sole carbon source, it’s maximum specific growth rate μmax＝0.21 hr-1, saturated constant Ks＝0.39 g/L and saturated inhibited constant Ki＝7.06 g/L. 82 hours fermentation get maximum cell concentration 6.4 g/L. PH-2 strain cultivated with phenol as sole carbon source, it’s maximum specific growth rate μmax＝0.26 hr-1, saturated constant Ks＝148.26 mg/L and saturated inhibited constant Ki＝668.64 mg/L. 48 hours fermentation get maximum cell concentration 6.3 g/L. When the strains was cultivated to log phase, the fermentation broth blended with wheat brans to produce bioaugmentation agents by warm air-dry method. Diesel oil biodegraded by mixed bioaugmentation agents can get good degraded result. Diesel oil and C12-C22 can be degraded 80 % and 75 % in 7 days. Kerosene biodegraded by single KS-1 strain bioaugmentation agents and mixed bioaugmentation agents weren’t obvious different. Kerosene and C9-C14 can be degraded 60 % and 55 % in 7 days.