| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 90 === Alzheimer’s disease (AD) is characterized by the progressive neuron-degenerative disease which loss of neuron function. p53 and its downstream p21 protein expression related senesces, age-related disease and carcinogenesis with response to cell growth arrest, DNA damage and apoptosis. In the present study, we observed the morphology, cell count, tunnel method and DNA fragmentation after 10μM aging amyloid 1-42 to E18 hippocampus primary neuron culture. The morphology and cell viability showed no specific change and neuron loss. TUNEL analysis and DNA ladder showed no apoptosis express. But increase p53 and p21 protein expression at 24hr of primary neuron culture and 24hr of APP695 overexpression cell line. Besides, we analysis cell cycle of DNA content after amyloid treated neurons, the result showed increase G1 arrest level of primary neuron after treated amyloid and APP 695 cell line after serum stimulation. We interpret these finding, as a possible mechanism lead to amyloid exist increase neuron aging processes of Alzheimer’s disease.
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