Forensic applications of chloroplast DNA-trnL intron and trnL-trnF IGS

• Main Authors: Yung-Chien Yu, 余勇健
• Other Authors: Hsing-Mei Hsieh
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/mtk6ty

碩士 === 中央警察大學 === 鑑識科學研究所 === 90 === The intron of trnL(UAA) and intergenic spacer (IGS) of trnL(UAA) - trnF(GAA) in the chloroplast genome were used as phylogenetic makers in many researches. The sequence variances represent the relationship of evolution among each plant. This research collected 335 samples from common plants including 221 species of 72 families in north Taiwan. The trnL intron and trnL-trnF IGS of each sample was sequenced to assess phylogenetic analysis for forensic applications. The 55 of 65 species for trnL intron and the 62 of 66 species for trnL-trnF IGS conformed to the species which have been known in the phylogenetic analysis. The remaining 4 species could not refer to the known groups by combining analyses of trnL intron and trnL-trnF IGS. Also 2 of 25 genuses could not entirely refer to the identified genuses in the phylogenetic analyses. The genetic distance and bootstrap values in phylogenetic analysis could refer to the species and genus of unknown sample. This study indicated that the trnL intron and trnL-trnF IGS could be used as efficient phylogenetic markers for forensic sciences.