Characteristics for microspheres of phosphotydalcholine / polylactide-co-glycolide for protein delivery in vitro study

• Main Authors: Yu-Lin Tsai, 蔡育霖
• Other Authors: Wen-Bin Chen
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/95384523597913106928

碩士 === 中原大學 === 醫學工程研究所 === 90 === Abstract Phosphotydalcholine (PC) was blended with polylactide-co-glycolide (PLGA) during double (W/O/W) emulsion and solvent evaporation steps when preparing microspheres for protein delivery. This work focused on the protein profile, the possible interactions between PC and PLGA and their relates prior to PLGA being added with release profiles. The morphology for PC/PLGA microspheres showed smooth surface with less pinhole compared with that for PLGA ones. Lower protein encapsulation efficiency for PC/PLGA microspheres compared with PLGA ones was observed . It is interesting to find that encapsulation efficiency for PC/PLGA microspheres was related to polylactide (PLA) contents in PLGA matrix. The hydrophobic property of different PLA contents of PLGA and their interactions with PC could be attributed to difference in protein encapsulation efficiency. Linear and non-initial burst albumin release profiles were observed for PC/PLGA microspheres that were suitable for protein delivery. Keywords: phosphotydalcholine, polylactide-co-glycolide, protein delivery, microspheres and biomaterial