| Summary: | 碩士 === 國立中興大學 === 昆蟲學系 === 90 === In this study, we cloned a membrane form guanylyl cyclase gene from the head RNA of Bactrocera dorsalis (Hendel), named Bdmgc1. Bdmgc1 has 4763 bp which contains an open reading frame with 1,251 deduced residues. The deduced protein contains a signal transmembrane domain that divides the protein into an amino acid-terminal extracellular ligand binding domain and a carboxyl-terminal intracellular domain, which further divides in to a kinase homologous domain and a cyclase catalytic domain. Five conserved cysteine residues and four Asn-linked glycosylation sites were indentified in the extracellular ligand binding domain of Bdmgc1. The kinase homologous domain contains 22 out of 33 residues that are invariant or conserved in protein kinases and a likely ATP binding site─Gly-X-X-Gly-X-X-Gly. Phylogenetic analysis of BdMGC1 and other membrane form guanylyl cyclases suggests that BdMGC1 might be a member of natriuretic peptide receptors. Reverse transcription-polychain reaction analysis demonstrated that Bdmgc1 transcripts was present throughout life cycle of B. dorsalis, including egg, larva, pupa and adult stages, however there were higher expression levels in the larva and pupal stages than other stages. Bdmgc1 transcripts expressed throughout the embryo and larval development periods. In the pupal stage, Bdmgc1 experssed at the highest level in the pharate adults.
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