探討酵母菌Glycyl-tRNA合成酵素之生化活性
碩士 === 國立中央大學 === 生命科學研究所 === 90 === 英文摘要 In the yeast Saccharomyces cerevisiae, protein synthesis takes place in two subcellular compartments: the cytosol and the mitochondria. Each of these two compartments requires a complete set of tRNA synthetases...
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ndltd-TW-090NCU001050132015-10-13T10:09:52Z http://ndltd.ncl.edu.tw/handle/00950848662259268008 探討酵母菌Glycyl-tRNA合成酵素之生化活性 賴志昌 碩士 國立中央大學 生命科學研究所 90 英文摘要 In the yeast Saccharomyces cerevisiae, protein synthesis takes place in two subcellular compartments: the cytosol and the mitochondria. Each of these two compartments requires a complete set of tRNA synthetases for function. Generally, these two sets of tRNA synthetases are encoded by distinct nuclear genes. An recent report provided evidence that GRS1 encodes both the cytoplasmic and mitochondrial GlyRS activities, while GRS2 is a pseudogene. The specific aims of this thesis are to explore the biochemical activities of GlyRS1 and GlyRS2 and to further dissect the evolutionary relationship between these two homologous genes. Interestingly, although GRS2 is nonfuncuional in vivo, purified GlyRS2 , when used at high concentration, charges yeast tRNA at a significant rate. Moreover, GlyRS2 cannot discriminate between yeast and E.coli tRNAs, while GlyRS1 strongly prefers yeast tRNAs. Thus, our results provide an evolutionary basis that ancient GRS1and GRS2 encode the cytoplasmic and mitochondrial GlyRS activities, respectively. 王健家 2002 學位論文 ; thesis 57 zh-TW |
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碩士 === 國立中央大學 === 生命科學研究所 === 90 === 英文摘要
In the yeast Saccharomyces cerevisiae, protein synthesis takes place
in two subcellular compartments: the cytosol and the mitochondria. Each
of these two compartments requires a complete set of tRNA synthetases
for function. Generally, these two sets of tRNA synthetases are encoded
by distinct nuclear genes. An recent report provided evidence that GRS1
encodes both the cytoplasmic and mitochondrial GlyRS activities, while
GRS2 is a pseudogene. The specific aims of this thesis are to explore the
biochemical activities of GlyRS1 and GlyRS2 and to further dissect the
evolutionary relationship between these two homologous genes.
Interestingly, although GRS2 is nonfuncuional in vivo, purified GlyRS2 ,
when used at high concentration, charges yeast tRNA at a significant rate.
Moreover, GlyRS2 cannot discriminate between yeast and E.coli tRNAs,
while GlyRS1 strongly prefers yeast tRNAs. Thus, our results provide an
evolutionary basis that ancient GRS1and GRS2 encode the cytoplasmic
and mitochondrial GlyRS activities, respectively.
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王健家 |
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王健家 賴志昌 |
author |
賴志昌 |
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賴志昌 探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
author_sort |
賴志昌 |
title |
探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
title_short |
探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
title_full |
探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
title_fullStr |
探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
title_full_unstemmed |
探討酵母菌Glycyl-tRNA合成酵素之生化活性 |
title_sort |
探討酵母菌glycyl-trna合成酵素之生化活性 |
publishDate |
2002 |
url |
http://ndltd.ncl.edu.tw/handle/00950848662259268008 |
work_keys_str_mv |
AT làizhìchāng tàntǎojiàomǔjūnglycyltrnahéchéngjiàosùzhīshēnghuàhuóxìng |
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1716827125889105920 |