Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell
碩士 === 國立中山大學 === 生物科學系研究所 === 90 === Alpha-1-microglobulin is co-synthesized with bikunin on the AMBP gene (□-1m-bikunin precursor gene). The major synthesis site of this protein is liver. It is excreted into urine by pass through glomerulus. Both alpha-1-microglobulin and bikunin are regarded as i...
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ndltd-TW-090NSYS51120322015-10-13T10:26:51Z http://ndltd.ncl.edu.tw/handle/13173176619415806067 Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell 草酸對於腎細胞甲一型微球蛋白生成的影響 Jeng-Yu Tsai 蔡政諭 碩士 國立中山大學 生物科學系研究所 90 Alpha-1-microglobulin is co-synthesized with bikunin on the AMBP gene (□-1m-bikunin precursor gene). The major synthesis site of this protein is liver. It is excreted into urine by pass through glomerulus. Both alpha-1-microglobulin and bikunin are regarded as inhibitors of urolithiasis. This investigation is attempted to evaluate if mRNA in renal epithelium cell may be up-regulated by oxalate and oxalate crystal stimulation. LLC-PK1 cells were challenged with oxalate and calcium oxalate crystal. The messenger RNA was collected and reverse transcripted into cDNA. PCR and real time PCR methods using primer designed from rat alpha-1-microglobulin gene were performed to amplify cDNA in cellular samples. Western blot was used to monitor the amount of specific proteins produced in culture media and in cells. The result showed that cellular AMBP gene was up-regulated and alpha-1-microglobulin protein increased in culture media after treated with oxalate and calcium oxalate crystal. David, Chao 趙大衛 2002 學位論文 ; thesis 30 zh-TW |
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碩士 === 國立中山大學 === 生物科學系研究所 === 90 === Alpha-1-microglobulin is co-synthesized with bikunin on the AMBP gene (□-1m-bikunin precursor gene). The major synthesis site of this protein is liver. It is excreted into urine by pass through glomerulus. Both alpha-1-microglobulin and bikunin are regarded as inhibitors of urolithiasis. This investigation is attempted to evaluate if mRNA in renal epithelium cell may be up-regulated by oxalate and oxalate crystal stimulation. LLC-PK1 cells were challenged with oxalate and calcium oxalate crystal. The messenger RNA was collected and reverse transcripted into cDNA. PCR and real time PCR methods using primer designed from rat alpha-1-microglobulin gene were performed to amplify cDNA in cellular samples. Western blot was used to monitor the amount of specific proteins produced in culture media and in cells. The result showed that cellular AMBP gene was up-regulated and alpha-1-microglobulin protein increased in culture media after treated with oxalate and calcium oxalate crystal.
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author2 |
David, Chao |
author_facet |
David, Chao Jeng-Yu Tsai 蔡政諭 |
author |
Jeng-Yu Tsai 蔡政諭 |
spellingShingle |
Jeng-Yu Tsai 蔡政諭 Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
author_sort |
Jeng-Yu Tsai |
title |
Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
title_short |
Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
title_full |
Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
title_fullStr |
Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
title_full_unstemmed |
Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell |
title_sort |
expression of alpha-1-microglobulin mrna change after oxalate challenge in renal epithelium cell |
publishDate |
2002 |
url |
http://ndltd.ncl.edu.tw/handle/13173176619415806067 |
work_keys_str_mv |
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