Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells
碩士 === 國立清華大學 === 生命科學系 === 90 === Mammalian cells possess a remarkable repertoire of responses to diverse stresses, particularly those inflicting genetic stability. UV, which is well known to generate photoproducts and the pyrimidine dimer may arrest cell cycle progression for DNA repai...
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ndltd-TW-090NTHU01050502015-10-13T10:34:05Z http://ndltd.ncl.edu.tw/handle/48164520258613203822 Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells 中國倉鼠卵巢細胞受紫外線誘導及其被秋水仙素加成的細胞死亡的特性 Yi-Ying Wu 吳奕瑩 碩士 國立清華大學 生命科學系 90 Mammalian cells possess a remarkable repertoire of responses to diverse stresses, particularly those inflicting genetic stability. UV, which is well known to generate photoproducts and the pyrimidine dimer may arrest cell cycle progression for DNA repair or lead to apoptosis by evoking checkpoint pathway. Previous researches in our laboratory has found that CHO.K1 cells, deficient in expression of p21 WAF1/CIP1, display UVC-induced cell death, which is promoted by the presence of anti-mitotic drugs in the cells following UVC irradiation. Here, we describe some phenomena or mechanisms to elucidate the cell death in advance. In CHO.K1 cells, UVC-induced cell death which is exacerbated in presence of colcemid (a mitotic inhibitor) belongs to apoptosis. UVC-induced DNA damage, neither ROS (Liao, 2001) nor death receptor activation, contributes to UVC-induced apoptosis. Although p21 WAF1/CIP1 is deficient in CHO.K1 cells, the cells exhibit normal nucleotide excision repair of UVC-induced DNA damage. However, the massive UVC-induced apoptosis in CHO.K1 cells is still due to the defect in p21waf1/cip1 so that CDK activity is not inhibited. During colcemid-promoted apoptosis following UVC irradiation, neither decrease of mitochondrial membrane potential nor release of Ca2+ in cytosol is observed. Over-activation of cdk1 and delay of DNA repair may result in the promoted apoptosis by colcemid. Yin-Chang Liu 劉銀樟 2002 學位論文 ; thesis 62 en_US |
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碩士 === 國立清華大學 === 生命科學系 === 90 === Mammalian cells possess a remarkable repertoire of responses to diverse stresses, particularly those inflicting genetic stability. UV, which is well known to generate photoproducts and the pyrimidine dimer may arrest cell cycle progression for DNA repair or lead to apoptosis by evoking checkpoint pathway. Previous researches in our laboratory has found that CHO.K1 cells, deficient in expression of p21 WAF1/CIP1, display UVC-induced cell death, which is promoted by the presence of anti-mitotic drugs in the cells following UVC irradiation. Here, we describe some phenomena or mechanisms to elucidate the cell death in advance. In CHO.K1 cells, UVC-induced cell death which is exacerbated in presence of colcemid (a mitotic inhibitor) belongs to apoptosis. UVC-induced DNA damage, neither ROS (Liao, 2001) nor death receptor activation, contributes to UVC-induced apoptosis. Although p21 WAF1/CIP1 is deficient in CHO.K1 cells, the cells exhibit normal nucleotide excision repair of UVC-induced DNA damage. However, the massive UVC-induced apoptosis in CHO.K1 cells is still due to the defect in p21waf1/cip1 so that CDK activity is not inhibited. During colcemid-promoted apoptosis following UVC irradiation, neither decrease of mitochondrial membrane potential nor release of Ca2+ in cytosol is observed. Over-activation of cdk1 and delay of DNA repair may result in the promoted apoptosis by colcemid.
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author2 |
Yin-Chang Liu |
author_facet |
Yin-Chang Liu Yi-Ying Wu 吳奕瑩 |
author |
Yi-Ying Wu 吳奕瑩 |
spellingShingle |
Yi-Ying Wu 吳奕瑩 Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
author_sort |
Yi-Ying Wu |
title |
Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
title_short |
Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
title_full |
Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
title_fullStr |
Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
title_full_unstemmed |
Characterization of UVC-induced cell death that is promoted by colcemid in CHO-K1 cells |
title_sort |
characterization of uvc-induced cell death that is promoted by colcemid in cho-k1 cells |
publishDate |
2002 |
url |
http://ndltd.ncl.edu.tw/handle/48164520258613203822 |
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