泡桐轉殖系統之建立

• Main Authors: shieh-cheng hang, 韓謝忱
• Other Authors: ya-nan wang
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/82760150112598865739

碩士 === 國立臺灣大學 === 森林學研究所 === 90 === Abstract Paulownia wood has been well utilized due to it’s light weight, rot resistance and free of warping. The fast growth rate of Paulownia may be capitalized upon for agroforestry. Paulownia fortunei is readily propagated through regeneration, transformed clonal lines could be bulked up from single shoots transformed with interest gene. In this report, we introduce the insect resistance gene, sporamin into Paulownia fortunei under 35S promoter control. The sporamin cDNA gene was constructed in a binary vector pBI121 at BamHI site, and then transformed Paulownia fortunei mediated by Agrobacterium tumerfaciens LBA4404.We also established the regeneration system and transformation system for transgenic Paulownia. Further used to transform the different tissue of Paulownia fortunei that selected by selection medium (kanamycin 30ppm), Twelve transgenic plantlets of them were analyzed by PCR analysis and Northern blot analysis. However, sporamin protein gene expression was investigated only in TP1, TP2, TP5, TP6, transgenic lines, but not in TP3, TP4, and TP6 after Western blot and trypsin inhibitory activity staining analysis. It suggested that a gene silencing might occur at the translational level in some transformants.