| Summary: | 碩士 === 國立臺灣大學 === 園藝學研究所 === 90 === Genetic Relationship between Ornamental Bromeliads of Centric South America and Commerical Cultivars in Taiwan.
The RAPD polymorphic markers were used to analyze the genetic similarity among eighteen collected accessions and cultivars attributed to seven genera of Bromeliaceae. A total of 119 markers were obtained from 20 RAPD primers. In genus identification, there are four, three, two, twelve, one, and one markers specific to Cryptanthus, Dyckia, Guzmznia, Neoregelia, Tillandsia, and Vriesea, respectively. Besides, all the eighteen tested accessions could be identified with thirty proposed markers. The result suggests that these thirty markers are useful tools for genera and species or cultivars discrimination, especially for species with high morphological similarity. The dendrogram established by Jaccard and UPGMA methods is coincident with the taxonomy. Moreover, our studies suggest a potential molecular marker to identify the cultivars of Bromeliaceae.
Somatic Embryogenesis in Cell Suspension Culture of Ornamental Bromeliads.
The various parts in young floret of ornamental bromeliad Guzmania cv. Cheery were used as explants inoculated on 1/2 MS medium supplemented with citric acid 0.1 mg/ml and 2,4-D 0.2-1.5 mg/L combinated with NAA 0.5 mg/L. The petal explants were potential to dedifferentiate embryogenic callus as high as 80 % by 2-3 months after culture. The callus with proembryoids were transferred into TB5 liquid medium added with 2,4-D 0.5 mg/L, cultured on 110 rpm shaker. The heterogeneous suspension cells were achieved about 2 months after suspension culture, which were competent to proliferate in apolar growth cycle, and formation of amorphic preembryogenic masses. The screened PEMs were plated on filter paper or gellified stratum enriched with half storgth basal salt of SH medium supplemented with NAA 0.2 mg/L, 2ip 0.2 mg/L and kinetin 0.1 mg/L, and induced multiple somatic embryo formation in each mini-callus. The mature somatic embryos were initially to in sprout, and acquired 200-400 shoots per dish inoculated with 1/30 PCV. The somatic embryos or emerging shoots were transferred on 1/2 SH medium after with NAA 0.2 mg/L and 2ip 0.2 mg/L benefit to converte into vigorous plantlets. The survival in exvitro transplant of the plantlets approached close to 100 % under mist propagation condition.
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