Production and Application of Melanin

• Main Authors: I-YEN Lee, 李奕彥
• Other Authors: Chien-Yuan Chen
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/11147378766131518259

碩士 === 國立臺灣大學 === 農業化學研究所 === 90 === It has long been believed that the formation of melanin in human skin is a protective response to the damaging effects of UV radiation and that melanin offers protection against futher UV-induced damage. There are indication that melanins are involved in free radical reactions of lipid peroxidation , where thy may act as natural radio- and photo- protectors. We have construction four plasmid (pLCJ-1 pLCJ-2 pLCJ-3 pLCJ-4), which have tyrosinase gene from Streptomyces sp which can produce melanin, and design an adaptive medium and expression situation for the best strain pLCJ-1, more distant we had design an adaptive fermentation situation for the strain. The medium is composed of sucrose 1%、NH4Cl 2%、tyrosine 1%、yeast extract 0.5%、Na2HPO4 0.05M, the adaptive fermentation situation is 1.5 vvm、37℃、initial pH 7.4、agitation rats 200 rpm、inoculum size 1%、inoculum age 6 hr. We also studied the antioxidant and anti-UV habitudes of melanin made from the gene transformants strain, and attempt to use the melanin we produced to explore it’s application in the antiradiation and anti-UV of mammalian cell. We find an additive of 0.1mg/ml melanin can protect mammalian cell from the damage of UV radiation，and an additive of 0.5mg/ml melanin can increase the resistance of mammalian cell in radioactivity.