| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 90 === The purpose of this research was to study the effects of high molecular weight
chitosan on lipid digestibility and intestinal physiological functions in
rats. Three chitosans ( Mv: 600、300 and 150 kDa ) were prepared from a
chitosan（1200 kDa）by enzymatic cleavage under pH 4 at 37 ℃. The degree of
deacetylation of these chitosan is approximately 80 %. Five groups of ten
Wistar rats were fed a high-fat diet ( 15 % soybean oil ) containing 5 %
cellulose (A) and 5 % chitosan with different molecular weight ( B：1200 kDa, C
：600 kDa, D：300 kDa, E：150 kDa ) for 4 weeks. Body weight and feed intake
were recorded daily. At the end of the experimental period, fecal and blood
samples were collected for the analysis of nutrient digestibility and lipid
profiles, respectively. When compared to control group, food intake, body
weight gain, epidiymal fat pad weight, apparent digestibility of fat and
protein were decreased significantly in the chitosan-fed groups. In addition,
total fecal weight, fecal moisture and lipid content, cecal wall weight were
increased by chitosan feeding. However, blood total cholesterol, LDL and HDL
concentrations were not affected by the addition of chitosan in feed. These
results suggest that intake of high molecular weight chitosan can prevent body
weight gain and body fat accumulation in rats under high-fat feeding condition.
|
|---|
