Effect of high molecular weight chitosan on nutrient digestibility and intestinal physiological function in rats

• Main Authors: Kuang-Lin Chen, 陳廣麟
• Other Authors: Kung-Chu Chan
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/08595948472503793131

碩士 === 靜宜大學 === 食品營養研究所 === 90 === The purpose of this research was to study the effects of high molecular weight chitosan on lipid digestibility and intestinal physiological functions in rats. Three chitosans ( Mv: 600、300 and 150 kDa ) were prepared from a chitosan（1200 kDa）by enzymatic cleavage under pH 4 at 37 ℃. The degree of deacetylation of these chitosan is approximately 80 %. Five groups of ten Wistar rats were fed a high-fat diet ( 15 % soybean oil ) containing 5 % cellulose (A) and 5 % chitosan with different molecular weight ( B：1200 kDa, C ：600 kDa, D：300 kDa, E：150 kDa ) for 4 weeks.　Body weight and feed intake were recorded daily. At the end of the experimental period, fecal and blood samples were collected for the analysis of nutrient digestibility and lipid profiles, respectively. When compared to control group, food intake, body weight gain, epidiymal fat pad weight, apparent digestibility of fat and protein were decreased significantly in the chitosan-fed groups. In addition, total fecal weight, fecal moisture and lipid content, cecal wall weight were increased by chitosan feeding. However, blood total cholesterol, LDL and HDL concentrations were not affected by the addition of chitosan in feed. These results suggest that intake of high molecular weight chitosan can prevent body weight gain and body fat accumulation in rats under high-fat feeding condition.