The Long-Term Effect of Folate-Deficient on Lipids Metabolism in Sprague-Dawley Rat

• Main Authors: Chia-Fang Tsai, 蔡佳芳
• Other Authors: Ming-Min Wei
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/51624121440529456495

碩士 === 靜宜大學 === 食品營養研究所 === 90 === The objective of this study was to investigate the effects of normal diet on lipid metabolism in the long-term folate-deficient rats. Weaning male Sprague-Dawley rats were fed an AIN-93G folate-deficient basal diet (folate deficient group) or a basal diet supplemented with 4.5μmol folate/kg diet (control group). To inhibit GI micro flora produced folate, 1% succinylsulfathiazole was added to the folate deficient group. The amount of food given, food spilled, and stool was measuring almost every day, and twice a week measured body weight and drinking water during the 16 weeks. At the end of feeding periods the rats were sacrificed. Compare folate deficient rats growth status, sulfa compounds, blood and liver lipids, choline, phosphatidylcholine, fatty acid profile, and lipogenic enzymes activities with control group. The results indicated that folate deficiency rats serum and liver folate concentration (13.3±2.6ng/mL, 1.0±0.1μg/g) significantly lower than control group (59.8±5.0ng/mL、2.7±0.3μg/g) (P＜0.05). Body weight in the rats fed folate deficient diets after 6 weeks (480.0±21.3g) and cumulative water consumed after 9 weeks (1569.1±285.5mL) were significantly lower than the control group (509.0±27.7g, 1940.9±364.5mL) (P＜0.05). Plasma homocysteine level of folate deficient group (33.3±6.7μM) was higher than control group (4.7±1.0μM) (P＜0.05). The SAH level of liver (10.6±2.7μmol/g) was increased in folate deficient group (5.9±1.2μmol/g) (P＜0.05). The liver SAM (1.9±2.0μmol/g) was decreased in folate deficient group (14.7±8.6μmol/g) (P＜0.05). The folate deficient diet did not influence plasma cysteine and glutathione level. When fed folate deficient diet serum triglyceride, total cholesterol, HDL, LDL, and phospholipid concentration was significantly lower than the control group (P＜0.05). This results was not cause to excreted fecel lipid or lipid in liver increase at folate deficient group. In addition, liver choline and phosphatidyl- choline level of folate deficient group (13.93±2.88μg/g, 73.36±8.51μg/g) was decreased than control group (23.25±8.13μg/g, 85.46±7.56μg/g) (P＜0.05). Moreover, folate deficient group had lower G6PDH activity (2.60±6.67 unit/mg protein) of liver than control group (23.05±11.63 unit/mg protein) (P＜0.05), while folate deficient diet didn’t influence ME and FAS activities of liver. However liver decosahexaenoic acid (C22:6) content (0.33±0.10mg/100g) was significantly lower than control group (0.51±0.13mg/100g) (P＜0.05). In addition, red blood cell, hemoglobin, and hematocrit was significantly decreased (P＜0.05), but MCV and MCH was higher than control group (P＜0.05); and further, white blood cell, monocyte, total and lymphocyte was lower than control group (P＜0.05). In conclusion, liver choline and phosphatidylcholine level was decreased in long-term folate deficiency rats. It might cause the blood lipid decreased. These results indicated that fed folate deficient diet might affect lipid oxidation to produce energy. While the G6PDH activity might inhibition. The mechanism is still unclear.