| Summary: | 碩士 === 台北醫學院 === 生物醫學技術研究所 === 90 === Some studies have demonstrated that cancer cell G2/M or G0/G1 cell cycle arrest and apoptosis were induced by antifungal drugs, griseofulvin (GF) or ketoconazole (KT). In this study, four kinds of antifungal drugs were chosen which include griseofulvin (GF), nystatin (NT), amphotericin B (AmB) and clotrimazole (CLT). To study the effects of these drugs on the growth of murine L-5178Y tumor cells, the percentage of survival tumor cells, cell apoptosis and cell cycle were observed. The former was measured by using trypan blue stain and the latter was analyzed by flow cytometry. In addition, DNA laddering was performed by using agarose gel electrophoresis and Bax protein were detected by western blot assay. In this study, different concentrations of each drug were dissolved in the culture medium. The doses of each drug were calculated in the effective range of GF (from 10mM to 80mM), AmB (from 1mM to 25mM), CLT (from 1mM to 25mM) and NT (from 1mM to 30mM). The results showed that GF、CLT and AmB revealed dose dependent relation on tumor cell apoptosis and viability. In addition, cell cycle arrest was induced by treating with GF or CLT, contrary, NT showed no effect on tumor cells. The results demonstrated that the antifungal drugs, griseofulvin, clotrimazole and amphotericin B may induce apoptosis in L-5178Y lymphoma cells and might to prove to be a novel therapy for lymphocytic cancer.
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