| Summary: | 碩士 === 台北醫學院 === 細胞及分子生物研究所 === 90 === Abstract
The highly proliferative potential colony - forming Assay (CFA) has been known as one of the most informative, reliable, and versatile short-term in vitro assay for examining stem/progenitor cell function. Chinese drugs working on hematopoietic system were usually examined by feeding mice with herb extract before or after irradiation damage of hematopoietic stem/progenitor cells (HS/PC) in bone marrow. In this study we use human cord blood HS/PC to conduct a human erythoid and myeloid colony assay (hCFA) for evaluation of Chinese herb function on blood renewing and immunity improving. Our results show that (1) the methanol extract of PG increases the size of the CFU-GM colonies significantly, and the cells are similar to the activated macrophage. (2) CSZ extract in 50% EtOH promotes cell proliferation of the CFU-GM. (3) The extract,SD, and ,SM, enhance both BFU-E and CFU-E formation. (4) The effects of chemical BJ-1, BJ-2, BJ-3, TWD-1 significantly inhibited the formation of BFU-E and CFU-E, however, TWD-1 inhibits the formation of BFU-E and CFU-E. (5) The extract, SC, at concentration 100μg/ml inhibits the all kind colony formation slightly ,but at the same concentration entirely inhibits the TF-1 cell proliferation. The above results suggest that the in vitro CFA method presented an advantage on economic and speed. In this study we concluded that changes in morphology, size, number of colony can be used to evaluate the function and toxicity of Chinese herb as well as the synthetic chemical drug candidates.
Key Words: Chinese Herb Function,In Vitro Colony Formation Assay,Hematopoietic Stem Cell
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