| Summary: | 碩士 === 大同大學 === 生物工程研究所 === 90 === Chromates are released into environment from wastewater and ash of manufactures for pigments, alloy, and leather tanning. Chromate usually causes the sickness in gastrointestinal tract, and is directly relative to the cancers of stomach and liver reported in nearly years. Pseudomonas putida CCRC 10459 was studied for the resistant and reduction of chromate in our laboratory for several years. In order to resolve the problem, the chromate reductase gene (chrR) was cloned from its genome and expressed in E. coli BL-21. The transformant, induced by IPTG for 2 hours, was cultured in LB medium with different concentrations of chromate ion. After 6 hours, all the concentrations of chromate are obviously reduced from the cultures with 50 to 1000M of chromate. The induced recombinant E. coli grows more poorly in the defined medium than in LB medium, but reduces more chromate at low concentration. On the other hand, the induced recombinant and Pseudomonas putida CCRC 10459 can reduce chromate continuously in aerobic culture, but only at the first hours in anaerobic culture.
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