| Summary: | 碩士 === 國立陽明大學 === 放射醫學科學研究所 === 90 === Prostate carcinoma is the common cancer among aged male population. In Taiwan, the incidence rate of prostate cancer increases annually and is ranked eighth in cancer mortality. Radiotherapy (RT) is the major treatment for prostate cancer. In this study, the variant frequencies (Vf) of hypothanthine phosphoribosyl transferase (HPRT) gene in lymphocytes of normal controls and prostate cancer patients post RT were assayed by immunofluorescence staining method, and the results were compared.
Seventeen normal donors, 67-84 years and average age 73.5 years, 8 males and 9 females, were used as controls in this study. The mean HPRT Vf of these normal controls was (0.93±0.49)×10-5. No significant difference (p = 0.065) was found between the mean Vf of males [ (1.16±0.53)×10-5] and that of females [(0.72±0.36)×10-5].
Thirty prostate cancer patients, 65-89 years and average age 74.5 years, the time periods post-RT 6 to 76 months, were. Studied. The mean Vf of all patients was (4.52±2.72)×10-5, which was about 5 folds higher than that of the normal controls (p < 0.001) and 4 folds higher than normal males (p = 0.002).
The increase of HPRT Vfs of post-RT cancer patients, was found with no correlation with age, radiation doses and the status of rectal bleeding. The HPRT Vf of post-RT cancer patients was not significantly reduced as the time period increased after radiotherapy. However, using three years as a cutoff time point (T ≦ 3 years and T > 3 years), the HPRT Vfs of the patients with T ≦ 3 years were decreased as the time increased (p < 0.001).
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