The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA
碩士 === 中國醫藥學院 === 中國藥學研究所 === 91 === Kinsenren(Anoectochilus formosanus HAYATA)is a Taiwanese Orchidaceae plant, which has been reported to exhibit hepatoprotective、blood pressure depression、blood fat depression、antidiabetic、antiinflammatory、antioxidant、antitumor and immune system enhancing activiti...
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ndltd-TW-091CMCH00490012015-10-13T16:56:28Z http://ndltd.ncl.edu.tw/handle/50119344900539663436 The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA 臺灣金線連成分-天麻素、天麻苷元及尿嘧啶之藥物動力學研究 chiang fu mei 江芙美 碩士 中國醫藥學院 中國藥學研究所 91 Kinsenren(Anoectochilus formosanus HAYATA)is a Taiwanese Orchidaceae plant, which has been reported to exhibit hepatoprotective、blood pressure depression、blood fat depression、antidiabetic、antiinflammatory、antioxidant、antitumor and immune system enhancing activities. For the pharmacokinetic and bioavailability studies, we select three active constituents of Kinsenren, including gastrodin、p-hydroxybenzylalcohol(HBA)and uracil as denoted constituents. A simple and sensitive HPLC method involving UV detection was developed for simultaneous determination of active three constituents of Kinsenren in preparations and plasma samples. A Mightysil-RP-18GP(4.6×250mm, 5μm)column with the mobile phase consisted of MeOH:0.1﹪H3PO3-H20=1:99 was used. The flow rate was 1.0 mL/min. The monitoring wavelength was 270 nm. Vadilation test give satisfied accuracy and precision for this method. After IV administration of the three constituents to rats, the pharmacokinetics of these three constituents in rats were well fitted to an open two-compartment model. The elimination half-life of gastrodin was 25.34 min, HBA was 85.45 min and uracil was 155.98 min. After oral administration of the three constituents to rats, the absolute bioavailability of gastrodin、HBA and uracil were 58.63﹪、80.83﹪and 41.07﹪respectively. The results suggest that repeated doses are necessary for Kinsenren in clinical use. 林文川 吳金濱 2003 學位論文 ; thesis 1 zh-TW |
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碩士 === 中國醫藥學院 === 中國藥學研究所 === 91 === Kinsenren(Anoectochilus formosanus HAYATA)is a Taiwanese Orchidaceae plant, which has been reported to exhibit hepatoprotective、blood pressure depression、blood fat depression、antidiabetic、antiinflammatory、antioxidant、antitumor and immune system enhancing activities. For the pharmacokinetic and bioavailability studies, we select three active constituents of Kinsenren, including gastrodin、p-hydroxybenzylalcohol(HBA)and uracil as denoted constituents.
A simple and sensitive HPLC method involving UV detection was developed for simultaneous determination of active three constituents of Kinsenren in preparations and plasma samples. A Mightysil-RP-18GP(4.6×250mm, 5μm)column with the mobile phase consisted of MeOH:0.1﹪H3PO3-H20=1:99 was used. The flow rate was 1.0 mL/min. The monitoring wavelength was 270 nm. Vadilation test give satisfied accuracy and precision for this method.
After IV administration of the three constituents to rats, the pharmacokinetics of these three constituents in rats were well fitted to an open two-compartment model. The elimination half-life of gastrodin was 25.34 min, HBA was 85.45 min and uracil was 155.98 min.
After oral administration of the three constituents to rats, the absolute bioavailability of gastrodin、HBA and uracil were 58.63﹪、80.83﹪and 41.07﹪respectively. The results suggest that repeated doses are necessary for Kinsenren in clinical use.
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林文川 |
author_facet |
林文川 chiang fu mei 江芙美 |
author |
chiang fu mei 江芙美 |
spellingShingle |
chiang fu mei 江芙美 The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
author_sort |
chiang fu mei |
title |
The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
title_short |
The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
title_full |
The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
title_fullStr |
The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
title_full_unstemmed |
The pharmacokinetic studies of Gastrodin, p-Hydroxybenzyl alcohol and Uracil-The constituents of Anoectochilus formosanus HAYATA |
title_sort |
pharmacokinetic studies of gastrodin, p-hydroxybenzyl alcohol and uracil-the constituents of anoectochilus formosanus hayata |
publishDate |
2003 |
url |
http://ndltd.ncl.edu.tw/handle/50119344900539663436 |
work_keys_str_mv |
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